Identification of Binding Epitopes of a Fluorinated Compound Bound to Proteins Using ¹H and ¹⁹F NMR Spectroscopy

• FURIHATA Kazuo

  Division of Agriculture and Agricultural Life Sciences, The University of Tokyo

• TASHIRO Mitsuru

  Department of Chemistry, College of Science and Technology, Meisei University

書誌事項

タイトル別名

• Identification of Binding Epitopes of a Fluorinated Compound Bound to Proteins Using ¹H and ¹⁹F NMR Spectroscopy

抄録

¹H/¹⁹F NMR-based screening methods were applied to a human serum albumin-fleroxacin complex. Fleroxacin contains three fluorine atoms in a molecule, which is suitable as a model fluorinated compound for NMR analysis with ¹H and ¹⁹F detection. The ¹⁹F{¹H} and ¹H{¹H} saturation transfer difference spectra were acquired and the ¹H/¹⁹F spin-lattice relaxation rates were measured with and without any selective irradiation of protein resonance to identify the binding epitopes of fleroxacin. Because several ¹H signals of fleroxacin resonated close to water, its precise signal intensities were unavailable. The ¹⁹F NMR-based screening methods successfully provide complementary information, indicating its importance in the analysis of fluorinated compounds.

収録刊行物

• Analytical Sciences

  Analytical Sciences 36 (7), 881-883, 2020-07-10

  社団法人 日本分析化学会

キーワード

• NMR-based screening
• fluorinated compounds
• ¹⁹F NMR

詳細情報

• CRID: 1390003825197506304
• NII論文ID: 130007872084
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.19n033
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 030505008
• PubMed: 32037346
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030505008
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.19N033.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.19N033/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/36/7/36_19N033/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2021182097
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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