Immunohistochemical Analysis of the Distribution of RANKL-Expressing Cells and the Expression of Osteoclast-Related Markers in Giant Cell Tumor of Bone
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- Shibuya Isao
- Department of Biochemistry, Showa University School of Dentistry Department of Orthopaedic Surgery, Teikyo University Mizonokuchi Hospital
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- Takami Masamichi
- Department of Pharmacology, Showa University School of Dentistry
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- Kawamoto Masashi
- Department of Diagnostic Pathology, Teikyo University Mizonokuchi Hospital
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- Karakawa Akiko
- Department of Pharmacology, Showa University School of Dentistry
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- Nakamura Shigeru
- Department of Orthopaedic Surgery, Teikyo University Mizonokuchi Hospital
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- Kamijo Ryutaro
- Department of Biochemistry, Showa University School of Dentistry
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Abstract
<p>To clarify the mechanism underlying the regulation of osteoclast differentiation and activation in giant cell tumor of bone, we investigated the expression of osteoclast-related markers in osteoclasts, neoplastic cells, and osteoclast precursors comprising giant cell tumor of bone using immunohistochemical analysis, and analyzed the distribution of receptor activator of nuclear factor κ-B ligand (RANKL)-expressing cells. We performed serial staining of sections using antibodies against osteoclast-related markers including RANKL, CD68, CD11b, c-Fms, RANK, and cathepsin K and analyzed the presence of the G34W mutation of H3F3A in giant cell tumor of bone. Mononuclear cells were CD11b+, CD68+, RANK+, and c-Fms+. In contrast, most multinucleate cells were CD11b–, CD68+, RANK+, and c-Fms+. RANKL was expressed in mononuclear cells and some multinucleate cells. Conversely, G34W was detected in mononuclear cells. RANKL+ mononuclear cells were also G34W+. RANKL expression was unevenly distributed in giant cell tumor of bone. RANKL+ cells were frequently localized along blood-containing cavity-like spaces. In areas with a high percentage of RANKL+ mononuclear cells, large numbers of osteoclasts were observed. In addition, the distribution of multinucleate cells (>100 µm in diameter) correlated with the distribution of RANKL+ cells. The distribution of RANKL+ mononuclear cells is uneven in giant cell tumor of bone and may have some effect on the localization of multinucleate cells. The distribution of mononuclear cells harboring the G34W mutation was identical to that of RANKL+ mononuclear cells.</p>
Journal
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- Journal of Hard Tissue Biology
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Journal of Hard Tissue Biology 29 (3), 137-146, 2020
THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
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Details 詳細情報について
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- CRID
- 1390566775153275520
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- NII Article ID
- 130007877464
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- NII Book ID
- AA11074332
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- ISSN
- 1880828X
- 13417649
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- NDL BIB ID
- 030573937
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed