Visualization of Procollagen IV Reveals ER-to-Golgi Transport by ERGIC-independent Carriers

• Matsui Yuto

  Laboratory of Molecular and Cellular Biology, Institute for Frontier Life and Medical Sciences, Kyoto University

• Hirata Yukihiro

  Laboratory of Molecular and Cellular Biology, Institute for Frontier Life and Medical Sciences, Kyoto University

• Wada Ikuo

  Department of Cell Sciences, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine

• Hosokawa Nobuko

  Laboratory of Molecular and Cellular Biology, Institute for Frontier Life and Medical Sciences, Kyoto University

Abstract

Collagen is the most abundant protein in animal tissues and is critical for their proper organization. Nascent procollagens in the endoplasmic reticulum (ER) are considered too large to be loaded into coat protein complex II (COPII) vesicles, which have a diameter of 60–80 nm, for exit from the ER and transport to the Golgi complex. To study the transport mechanism of procollagen IV, which generates basement membranes, we introduced a cysteine-free GFP tag at the N-terminus of the triple helical region of the α1(IV) chain (cfSGFP2-col4a1), and examined the dynamics of this protein in HT-1080 cells, which produce endogenous collagen IV. cfSGFP2-col4a1 was transported from the ER to the Golgi by vesicles, which were a similar size as small cargo carriers. However, mCherry-ERGIC53 was recruited to α₁-antitrypsin-containing vesicles, but not to cfSGFP2-col4a1-containing vesicles. Knockdown analysis revealed that Sar1 and SLY1/SCFD1 were required for transport of cfSGFP2-col4a1. TANGO1, CUL3, and KLHL12 were not necessary for the ER-to-Golgi trafficking of procollagen IV. Our data suggest that procollagen IV is exported from the ER via an enlarged COPII coat carrier and is transported to the Golgi by unique transport vesicles without recruitment of ER-Golgi intermediate compartment membranes. Key words: collagen, procollagen IV, endoplasmic reticulum, ER-to-Golgi transport, ERGIC

Journal

• Cell Structure and Function

  Cell Structure and Function 45 (2), 107-119, 2020

  Japan Society for Cell Biology

Keywords

• collagen
• procollagen IV
• endoplasmic reticulum
• ER-to-Golgi transport
• ERGIC

Details

• CRID: 1390848250131056768
• NII Article ID: 130007879591
• NII Book ID: AA0060007X
• DOI: 10.1247/csf.20025
• ISSN: 13473700
• ISSN: 03867196
• NDL BIB ID: 031311455
• PubMed: 32554938
• Web Site: http://id.ndl.go.jp/bib/031311455
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031311455
• Web Site: https://www.jstage.jst.go.jp/article/csf/45/2/45_20025/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2022034681
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed