Insulin Stimulation of Protein Synthesis and mTOR Signaling in Chick Myotube Cultures

  • Nakashima Kazuki
    Division of Animal Metabolism and Nutrition, Institute of Livestock and Grassland Science, NARO
  • Ishida Aiko
    Division of Animal Metabolism and Nutrition, Institute of Livestock and Grassland Science, NARO
  • Shimamoto Saki
    Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University
  • Ijiri Daichi
    Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University
  • Ohtsuka Akira
    Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University

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<p>Insulin stimulates protein synthesis in skeletal muscles. Protein synthesis is controlled by the mechanistic target of rapamycin (mTOR) signaling in skeletal muscles. This study was conducted to investigate the effect of insulin on protein synthesis and mTOR signaling in chick myotube cultures. Chick myotubes were incubated with insulin (1 µg/ml) for 1 h. Protein synthesis, measured using the surface sensing of translation method, was significantly increased by insulin. The phosphorylation of AKT (Thr308 and Ser473), p70 ribosomal S6 kinase 1 (S6K1, Thr389), S6 ribosomal protein (Ser235/236), and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1, Thr37/46) was also significantly increased by insulin. These results suggest that insulin stimulates protein synthesis via mTOR signaling (phosphorylation of AKT, S6K1, S6 ribosomal protein, and 4E-BP1) in chick myotube cultures.</p>

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