Development and Testing of an Individual Kernel Detection System for Genetically Modified Soybean Events in Non-identity-preserved Soybean Samples

• Soga Keisuke

  National Institute of Health Sciences

• Kimata Shinya

  National Institute of Health Sciences

• Narushima Jumpei

  National Institute of Health Sciences

• Sato Sakiko

  Kyoritsu Women’s University

• Sato Emi

  Kyoritsu Women’s University

• Mano Junichi

  National Agriculture and Food Research Organization

• Takabatake Reona

  National Agriculture and Food Research Organization

• Kitta Kazumi

  National Agriculture and Food Research Organization

• Kawakami Hiroshi

  Kyoritsu Women’s University

• Akiyama Hiroshi

  National Institute of Health Sciences

• Kondo Kazunari

  National Institute of Health Sciences

• Nakamura Kosuke

  National Institute of Health Sciences

Bibliographic Information

Other Title

• Highlighted Paper selected by Editor-in-Chief : Development and Testing of an Individual Kernel Detection System for Genetically Modified Soybean Events in Non-identity-preserved Soybean Samples

Abstract

A genetically modified (GM) soybean kernel detection system using combination of DNA preparation from individual soybean kernels and event-specific real-time PCR was developed to simultaneously identify GM soybean events authorized for food after safety assessments in Japan. Over 100 kernels in the non-identity-preserved soybean samples imported from the United States of America (two U.S.A. lots) and Brazil (one lot) were randomly selected and examined. In total, 98 and 96% of the two independent U.S.A. lots, and 100% of the Brazilian lot contained GM soybean kernels. Herbicide-tolerant events, MON89788 (trade name Genuity^® Roundup Ready 2 Yield™), GTS 40-3-2 (trade name Roundup Ready™ soybean) and A2704-12 (trade name Liberty Link^® soybean), were detected similarly in both U.S.A. lots. In the Brazilian lot, in addition to GTS 40-3-2, a stacked GM event, MON87701 × MON89788, having insect-resistance and herbicide-tolerance, was detected. There were no unauthorized GM soybeans comingled, and the ratio of GM soybean events detected was consistent with statistical reports on the cultivated GM soybean events in both countries.

Journal

• Biological and Pharmaceutical Bulletin

  Biological and Pharmaceutical Bulletin 43 (8), 1259-1266, 2020-08-01

  The Pharmaceutical Society of Japan

Keywords

• genetically modified (GM)
• non-identity-preserved (non-IP)
• soybean
• real-time PCR
• event identification
• kernel

Details

• CRID: 1390003825203498752
• NII Article ID: 130007883796
• NII Book ID: AA10885497
• DOI: 10.1248/bpb.b20-00382
• ISSN: 13475215
• ISSN: 09186158
• NDL BIB ID: 030547150
• PubMed: 32741947
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030547150
• Web Site: https://www.jstage.jst.go.jp/article/bpb/43/8/43_b20-00382/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed