Direct evaluation of the antioxidant properties of salivary proline-rich proteins

• Komatsu Tomoko

  Division of Dentistry for the Special Patient, Department of Critical Care Medicine and Dentistry, Kanagawa Dental University Graduate School of Dental Medicine Yokosuka-Shonan Disaster Oral Health Research Center & Oxidative Stress/ESR Laboratories, Kanagawa Dental University Graduate School of Dental Medicine

• Kobayashi Kyo

  Yokosuka-Shonan Disaster Oral Health Research Center & Oxidative Stress/ESR Laboratories, Kanagawa Dental University Graduate School of Dental Medicine

• Morimoto Yoshinari

  Department of Critical Care Medicine and Dentistry, Kanagawa Dental University Graduate School of Dental Medicine

• Helmerhorst Eva

  Department of Molecular and Cell Biology, Boston University Henry M. Goldman School of Dental Medicine

• Oppenheim Frank

  Department of Molecular and Cell Biology, Boston University Henry M. Goldman School of Dental Medicine

• Chang-il Lee Masaichi

  Yokosuka-Shonan Disaster Oral Health Research Center & Oxidative Stress/ESR Laboratories, Kanagawa Dental University Graduate School of Dental Medicine

抄録

Proline-rich proteins are associated with the formation of an acquired protein layer overlying the tooth enamel surface. Previous studies have described the antioxidant activity of salivary histatin against the hydroxyl radical from Fenton’s reaction, acting as the critical reactive oxygen species. However, the role of proline-rich proteins in mitigating the oxidative stress caused by reactive oxygen species in the oral cavity remains unclear. In this study, we investigated the antioxidant effects of proline-rich proteins 2 on direct reactive oxygen species using electron spin resonance spectroscopy. For the first time, we demonstrated that proline-rich proteins 2 exhibits antioxidant activity directly against the hydroxyl radical produced by hydrogen peroxide with ultraviolet. Considering that identical results were obtained when assaying 30 residues of proline-rich proteins 2, the direct antioxidant effects against the hydroxyl radical by proline-rich proteins 2 may be related to these specific 30 residues.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ 67 (2), 131-136, 2020

  一般社団法人　日本酸化ストレス学会

キーワード

• saliva
• proline-rich proteins
• oxidative stress
• reactive oxygen species
• antioxidant

詳細情報

• CRID: 1390848250140409600
• NII論文ID: 130007894918
• DOI: 10.3164/jcbn.19-75
• ISSN: 18805086
• ISSN: 09120009
• Web Site: https://www.jstage.jst.go.jp/article/jcbn/67/2/67_19-75/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2021198735
• 本文言語コード: en
• データソース種別:
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