Inhibition of Devitrification on Cells Frozen without a Cryoprotectant Agent for a Long-term Storage
-
- WATANABE Hiroki
- Graduate School of Science and Technology, Shinshu University
-
- TAKIZAWA Hideyo
- Graduate School of Science and Technology, Shinshu University
-
- AKIYAMA Yoshitake
- Graduate School of Science and Technology, Shinshu University
Bibliographic Information
- Other Title
-
- 凍結保護剤フリーで凍結された細胞の長期保存に向けた脱ガラス化の抑制
- トウケツ ホゴザイ フリー デ トウケツ サレタ サイボウ ノ チョウキ ホゾン ニ ムケタ ダツガラスカ ノ ヨクセイ
Search this article
Abstract
Cryoprotectant agents (CPA) that can suppress generating ice crystals during the freezing process are essential for cell cryopreservation. On the other hand, a CPA should ideally be avoided due to their cytotoxicity and potential side effects. We previously reported a novel cryopreservation method without a CPA by ultrarapid cooling. In the method, cells were ejected as tiny droplets by inkjet cell printing. The droplets were deposited on the liquid nitrogen-cooled substrate and were vitrified, that enables cell cryopreservation. While, these droplets can devitrify easily due to CPA-free and low thermal volume of the droplets. In this study, we fabricated an aluminum career and a silicon cover to inhibit devitrification of the droplets. The experimental results indicated that the glass substrate with the career and the cover was kept under the glass-transition temperature for a several seconds. The efficacy of the career and the cover was also evaluated on mouse fibroblast 3T3 cells. The cells transferred to the cryotube and stored for one week with the career and the cover had higher viability than the ones without them. These results indicate that the career and the cover were able to inhibit devitrification of the cells frozen by our method.
Journal
-
- Cryobiology and Cryotechnology
-
Cryobiology and Cryotechnology 66 (1), 41-44, 2020
Japanese Society of Cryobiology and Cryotechnology
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390848250140473600
-
- NII Article ID
- 130007895012
-
- NII Book ID
- AN10448734
-
- ISSN
- 24241555
- 13407902
-
- NDL BIB ID
- 030457409
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- NDL
- CiNii Articles
- KAKEN
-
- Abstract License Flag
- Disallowed