Vestiges of Adaptation to the Mesophilic Environment in the Genome of <i>Tepiditoga spiralis</i> gen. nov., sp. nov.

• Mori Koji

  NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE)

• Sakurai Kenta

  NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE)

• Hosoyama Akira

  NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE)

• Kakegawa Takeshi

  Tohoku University

• Hanada Satoshi

  Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Graduate School of Science and Engineering, Tokyo Metropolitan University

Bibliographic Information

Other Title

• Vestiges of Adaptation to the Mesophilic Environment in the Genome of Tepiditoga spiralis gen. nov., sp. nov

Abstract

A novel anaerobic heterotrophic strain, designated strain sy52^T, was isolated from a hydrothermal chimney at Suiyo Seamount in the Pacific Ocean. A 16S rRNA gene analysis revealed that the strain belonged to the family Petrotogaceae in the phylum Thermotogae. The strain was mesophilic with optimum growth at 48°C and the phylum primarily comprised hyperthermophiles and thermophiles. Strain sy52^T possessed unique genomic characteristics, such as an extremely low G+C content and 6 copies of rRNA operons. Genomic analyses of strain sy52^T revealed that amino acid usage in the predicted proteins resulted from adjustments to mesophilic environments. Genomic features also indicated independent adaptions to the mesophilic environment of strain sy52^T and Mesotoga species, which belong to the mesophilic lineage in the phylum Thermotogae. Based on phenotypic and phylogenetic evidence, strain sy52^T is considered to represent a novel genus and species in the family Petrotogaceae with the proposed name Tepiditoga spiralis gen. nov., sp. nov.

Journal

• Microbes and Environments

  Microbes and Environments 35 (4), n/a-, 2020

  Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Keywords

• Thermotogae
• Tepiditoga spiralis
• Mesophilic environment

Details

• CRID: 1390004222620438784
• NII Article ID: 130007905730
• NII Book ID: AA11551577
• DOI: 10.1264/jsme2.me20046
• ISSN: 13474405
• ISSN: 13426311
• NDL BIB ID: 031205635
• PubMed: 32963207
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031205635
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/35/4/35_ME20046/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed