Anti-bacterial Effects of MnO₂ on the Enrichment of Manganese-oxidizing Bacteria in Downflow Hanging Sponge Reactors

• Matsushita Shuji

  Department of Civil and Environmental Engineering, Graduate School of Advanced Science and Engineering, Hiroshima University Western Region Industrial Research Center, Hiroshima Prefectural Technology Research Institute

• Hiroe Takafumi

  Department of Civil and Environmental Engineering, Graduate School of Advanced Science and Engineering, Hiroshima University

• Kambara Hiromi

  Department of Civil and Environmental Engineering, Graduate School of Advanced Science and Engineering, Hiroshima University

• Shoiful Ahmad

  Department of Civil and Environmental Engineering, Graduate School of Advanced Science and Engineering, Hiroshima University Center of Technology for the Environment, Agency for the Assessment and Application of Technology

• Aoi Yoshiteru

  Environmental Microbiology Laboratory, Graduate School of Advance Sciences of Matter, Hiroshima University

• Kindaichi Tomonori

  Department of Civil and Environmental Engineering, Graduate School of Advanced Science and Engineering, Hiroshima University

• Ozaki Noriatsu

  Department of Civil and Environmental Engineering, Graduate School of Advanced Science and Engineering, Hiroshima University

• Imachi Hiroyuki

  Department of Subsurface Geobiological Analysis and Research, Japan Agency for Marine-Earth Science & Technology

• Ohashi Akiyoshi

  Department of Civil and Environmental Engineering, Graduate School of Advanced Science and Engineering, Hiroshima University

Bibliographic Information

Other Title

• Anti-bacterial Effects of MnO₂ on the Enrichment of Manganese-oxidizing Bacteria in Downflow Hanging Sponge Reactors

Abstract

We focused on the use of abiotic MnO₂ to develop reactors for enriching manganese-oxidizing bacteria (MnOB), which may then be used to treat harmful heavy metal-containing wastewater and in the recovery of useful minor metals. Downflow hanging sponge (DHS) reactors were used under aerobic and open conditions to investigate the potential for MnOB enrichment. The results of an experiment that required a continuous supply of organic feed solution containing Mn(II) demonstrated that MnOB enrichment and Mn(II) removal were unsuccessful in the DHS reactor when plain sponge cubes were used. However, MnOB enrichment was successful within a very short operational period when sponge cubes initially containing abiotic MnO₂ were installed. The results of a microbial community analysis and MnOB isolation revealed that MnOB belonging to Comamonadaceae or Pseudomonas played a major role in Mn(II) oxidation. Successful MnOB enrichment was attributed to several unidentified species of Chitinophagaceae and Gemmataceae, which were estimated to be intolerant of MnO₂, being unable to grow on sponge cubes containing MnO₂. The present results show that MnO₂ exerted anti-bacterial effects and inhibited the growth of certain non-MnOB groups that were intolerant of MnO₂, thereby enabling enriched MnOB to competitively consume more substrate than MnO₂-intolerant bacteria.

Journal

• Microbes and Environments

  Microbes and Environments 35 (4), n/a-, 2020

  Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Keywords

• anti-bacterial effects
• biological manganese oxidation
• biological manganese oxide
• downflow hanging sponge reactor
• metal oxide

Details

• CRID: 1390567172573970432
• NII Article ID: 130007905875
• NII Book ID: AA11551577
• DOI: 10.1264/jsme2.me20052
• ISSN: 13474405
• ISSN: 13426311
• NDL BIB ID: 031205626
• PubMed: 32963206
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031205626
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/35/4/35_ME20052/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed