Comparative analysis of bovine serum albumin adsorption onto octacalcium phosphate crystals prepared using different methods
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- TSUCHIYA Kaori
- Division of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry
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- HAMAI Ryo
- Division of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry
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- SAKAI Susumu
- Division of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry
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- SUZUKI Osamu
- Division of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry
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Abstract
<p>This study compared bovine serum albumin (BSA) adsorption onto octacalcium phosphate (OCP) materials prepared from two wet preparations in the absence (w-OCP) and presence (c-OCP) of gelatin. Raman spectroscopy was used to analyze the BSA adsorption onto OCPs in a 150 mM Tris-HCl buffer containing 0.5 mM calcium and inorganic phosphate (Pi) ions at pH 7.4 and at 37°C. The degree of supersaturation of the supernatants after the adsorption was determined by measuring the ion composition. The results showed that BSA adsorption onto w-OCP was higher than that for c-OCP. The calcium ion concentration of the supernatant decreased for both w-OCP and c-OCP, whereas the Pi ion concentration increased, approaching OCP equilibria at different saturation levels. BSA adsorbed even onto c-OCP, which included a small amount of gelatin during c-OCP preparation. These results indicate that the biodegradability of w-OCP and c-OCP may be modulated through interactions with serum proteins.</p>
Journal
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- Dental Materials Journal
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Dental Materials Journal 39 (5), 883-891, 2020-09-28
The Japanese Society for Dental Materials and Devices