RNA virome analysis of hematophagous Chironomoidea flies (Diptera: Ceratopogonidae and Simuliidae) collected in Tokyo, Japan

DOI Web Site Web Site 2 Citations 10 References Open Access
  • Kobayashi Daisuke
    Department of Medical Entomology, National Institute of Infectious Diseases
  • Murota Katsunori
    Department of Medical Entomology, National Institute of Infectious Diseases Kyushu Research Station, National Institute of Animal Health, NARO
  • Faizah Astri Nur
    Department of Medical Entomology, National Institute of Infectious Diseases
  • Amoa-Bosompem Michael
    Department of Medical Entomology, National Institute of Infectious Diseases
  • Higa Yukiko
    Department of Medical Entomology, National Institute of Infectious Diseases
  • Hayashi Toshihiko
    Department of Medical Entomology, National Institute of Infectious Diseases
  • Tsuda Yoshio
    Department of Medical Entomology, National Institute of Infectious Diseases
  • Sawabe Kyoko
    Department of Medical Entomology, National Institute of Infectious Diseases
  • Isawa Haruhiko
    Department of Medical Entomology, National Institute of Infectious Diseases

Search this article

Abstract

<p>The development of sequencing technologies, in recent years, gives novel insights into the diversity of viruses in arthropods. Human pathogenic or possible pathogenic arthropod-borne viruses (arboviruses) including novel viruses from mosquitoes and ticks have been found by RNA virome analysis using a high-throughput sequencer. However, virome studies for other blood-sucking arthropods like biting midges as well as black flies are relatively scarce. In this study, to find viruses in hematophagous Chironomoidea flies, we performed RNA virome analyses of field-caught female Culicoides arakawae and Simulium aureohirtum as a pilot study. In the analyses, six novel viruses belonging to five virus taxa were detected, showing that RNA virome analysis using the next-generation sequencer was a strong method for understanding the viruses in both biting midges and black flies. This study indicated that C. arakawae and S. aureohirtum, which are not a popular vector for human pathogenic viruses, have a variety of viruses which are as many as other important vectors including mosquitoes and ticks. Furthermore, RNA virome analysis of a variety of blood-sucking insects will aid in not only discovering novel arboviruses but also understanding novel importance for arboviral vectors.</p>

Journal

Citations (2)*help

See more

References(10)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top