Spectroscopic and structural characteristics of a dual-light sensor protein, PYP-phytochrome related protein

• Sum Jia-Siang

  Division of Materials Science, Graduate School of Science and Technology, Nara Institute of Science and Technology

• Yamazaki Yoichi

  Division of Materials Science, Graduate School of Science and Technology, Nara Institute of Science and Technology

• Yoshida Keito

  Division of Materials Science, Graduate School of Science and Technology, Nara Institute of Science and Technology

• Yonezawa Kento

  Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK)

• Hayashi Yugo

  Division of Materials Science, Graduate School of Science and Technology, Nara Institute of Science and Technology

• Kataoka Mikio

  Division of Materials Science, Graduate School of Science and Technology, Nara Institute of Science and Technology

• Kamikubo Hironari

  Division of Materials Science, Graduate School of Science and Technology, Nara Institute of Science and Technology Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK)

Abstract

PYP-phytochrome related (Ppr) protein contains the two light sensor domains, photoactive yellow protein (PYP) and bacteriophytochrome (Bph), which mainly absorb blue and red light by the chromophores of p-coumaric acid (pCA) and biliverdin (BV), respectively. As a result, Ppr has the ability to photoactivate both domains together or separately. We investigated the photoreaction of each photosensor domain under different light irradiation conditions and clarified the inter-dependency between these domains. Within the first 10 s of blue light illumination, Ppr (Holo-Holo-Ppr) accompanied by both pCA and BV demonstrated spectrum changes reflecting PYP_L accumulation, which can also be observed in Ppr containing only pCA (Holo-Apo-Ppr), and a fragment of Ppr lacking the C-terminal Bph domain. Although Holo-Apo-Ppr showed PYP_L as a major photoproduct under blue light, as seen in the Bph-truncated Ppr, the equilibrium in the Holo-Holo-Ppr was shifted from PYP_L to PYP_M as the reaction progresses under blue light. Concomitantly, the spectrum of Bph exhibited subtle but distinguishable alteration. Together with the fact, it can be proposed that Bph with BV influences the photoreaction of PYP in Ppr, and vice versa. SAXS measurements revealed substantial tertiary structure changes in Holo-Holo-Ppr under continuous blue light irradiation within the first 5 min time domain. Interestingly, the changes in tertiary structure were partially suppressed by photoactivation of the Bph domain. These observations indicate that the photoreactions of the PYP and Bph domains are coupled with each other, and that the interplay realizes the structural switch, which might be involved in downstream signal transduction.

Journal

• Biophysics and Physicobiology

  Biophysics and Physicobiology 17 (0), 103-112, 2020

  The Biophysical Society of Japan

Keywords

• bacteriophytochrome
• photoactive yellow protein
• photoreactions
• small-angle x-ray scattering

Details

• CRID: 1390567172583120256
• NII Article ID: 130007919820
• DOI: 10.2142/biophysico.bsj-2020015
• ISSN: 21894779
• Web Site: https://www.jstage.jst.go.jp/article/biophysico/17/0/17_BSJ-2020015/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2022006350
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed