始原的呼吸機構の遺伝学的解析に向けた<i>Parageobacillus thermoglucosidasius</i>における一酸化炭素酸化能欠失株の創出とその性状

• Adachi Yuka

  Graduate School of Agriculture, Kyoto University

• Inoue Masao

  Graduate School of Agriculture, Kyoto University

• Yoshida Takashi

  Graduate School of Agriculture, Kyoto University

• Sako Yoshihiko

  Graduate School of Agriculture, Kyoto University

書誌事項

タイトル別名

• Genetic Engineering of Carbon Monoxide-dependent Hydrogen-producing Machinery in <i>Parageobacillus thermoglucosidasius</i>
• Genetic Engineering of Carbon Monoxide-dependent Hydrogen-producing Machinery in Parageobacillus thermoglucosidasius

抄録

The metabolic engineering of carbon monoxide (CO) oxidizers has the potential to create efficient biocatalysts to produce hydrogen and other valuable chemicals. We herein applied markerless gene deletion to CO dehydrogenase/energy-converting hydrogenase (CODH/ECH) in the thermophilic facultative anaerobe, Parageobacillus thermoglucosidasius. We initially compared the transformation efficiency of two strains, NBRC 107763^T and TG4. We then disrupted CODH, ECH, and both enzymes in NBRC 107763^T. The characterization of growth in all three disruptants under 100% CO demonstrated that both enzymes were essential for CO-dependent growth with hydrogen production in P. thermoglucosidasius. The present results will become a platform for the further metabolic engineering of this organism.

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 35 (4), n/a-, 2020

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• hydrogen
• carbon monoxide
• water-gas shift reaction
• markerless gene deletion
• <i>Parageobacillus</i>

詳細情報

• CRID: 1390286426513452160
• NII論文ID: 130007977127
• NII論文ID: 130007928698
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me20101
• DOI: 10.14862/geochemproc.67.0_179
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 031205555
• PubMed: 33087627
• Web Site: http://id.ndl.go.jp/bib/031205555
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031205555
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/35/4/35_ME20101/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可