Cell Density-dependent Anammox Activity of Candidatus Brocadia sinica Regulated by N-acyl Homoserine Lactone-mediated Quorum Sensing

• Oshiki Mamoru

  Division of Environmental Engineering, Faculty of Engineering, Hokkaido University

• Hiraizumi Haruna

  Division of Environmental Engineering, Faculty of Engineering, Hokkaido University

• Satoh Hisashi

  Division of Environmental Engineering, Faculty of Engineering, Hokkaido University

• Okabe Satoshi

  Division of Environmental Engineering, Faculty of Engineering, Hokkaido University

書誌事項

タイトル別名

• Cell Density-dependent Anammox Activity of <i>Candidatus</i> Brocadia sinica Regulated by <i>N</i>-acyl Homoserine Lactone-mediated Quorum Sensing

抄録

The activity of anaerobic ammonia-oxidizing (anammox) bacteria is considered to depend on cell density; however, this has not yet been confirmed due to the fastidious nature of anammox bacteria (e.g., slow growth, oxygen sensitivity, and rigid aggregate formation). In the present study, the cell density-dependent occurrence of anammox activity (^14-15N₂ gas production rate) was investigated using planktonic enrichment cultures of Candidatus Brocadia sinica. This activity was detectable when the density of cells was higher than 10⁷‍ ‍cells‍ ‍mL^–1 and became stronger with increases in cell density. At the cell densities, the transcription of the BROSI_A1042 and BROSI_A3652 genes, which are potentially involved in the biosynthesis and reception of N-acyl homoserine lactone (AHL), was detectable in Brocadia sinica cells. The presence of AHL molecules in the MBR culture of B. sinica was confirmed by an AHL reporter assay and gas chromatography mass spectrometry analysis. The exogenous addition of the MBR culture extract and AHL molecules (a cocktail of C₆, C₈, C₁₀, and C₁₂-homoserine lactones) increased the specific ^14-15N₂ production rate of B. sinica. These results suggest that the specific anammox activity of B. sinica is regulated by AHL-mediated quorum sensing.

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 35 (4), n/a-, 2020

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• anaerobic ammonium oxidation (anammox)
• <i>Ca.</i> Brocadia sinica
• cell density-dependent regulation
• <i>N</i>-acyl homoserine lactone
• quorum sensing

詳細情報

• CRID: 1391693801397047296
• NII論文ID: 130007929929
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me20086
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 031205530
• PubMed: 33100282
• Web Site: http://id.ndl.go.jp/bib/031205530
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031205530
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/35/4/35_ME20086/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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