The Induction of Heat Shock Protein 70 after Oral Administration of Concentrated Brewed Rice Vinegar Kurozu in Mice

• ARAKI Yuri

  Department of Basic Veterinary Science, Joint Faculty of Veterinary Medicine, Kagoshima University

• IDA Yuta

  Department of Basic Veterinary Science, Joint Faculty of Veterinary Medicine, Kagoshima University

• NONAKA Miwa

  Department of Basic Veterinary Science, Joint Faculty of Veterinary Medicine, Kagoshima University

• YOSHIZAKI Yumiko

  Education and Research Center for Fermentation Studies, Faculty of Agriculture, Kagoshima University

• FUJII Akira

  Sakamoto Kurozu, Inc.

• NAGANO Masanobu

  Sakamoto Kurozu, Inc.

• KANOUCHI Hiroaki

  Department of Clinical Nutrition, Osaka Prefecture University

抄録

Heat shock protein 70 (HSP70) is induced by various stresses. Since HSP70 has a protein refolding activity and an anti-inflammatory activity, the HSP70 induction will help cells from harmful acute stresses. Feeding a diet containing concentrated brewed rice vinegar Kurozu (CK) diet for 5 wk resulted in an increase of HSP70 in the brains of mice. In the present study, we evaluated whether oral feeding of 25 μL CK induces HSP70 mRNA in brain and other tissues. HSP70 mRNA was significantly increased in the esophagus, small intestine, liver, and brown adipose tissue within 1 h after the oral administration of CK. A weaker induction of HSP70 mRNA was demonstrated in the stomach, large intestine, and brain. HSP70 mRNA induction returned to basal levels within 3 h after feeding. We doubted that the induction of HSP70 mRNA was caused by manual restraint of the mice during CK administration. Manual restraint of the mice did not influence HSP70 mRNA expression in intestine 1 h after these treatments. Our results suggest that transient HSP70 mRNA induction by oral feeding of CK was not caused by retention stress. There are some compounds in CK that increase HSP70 mRNA in various tissues.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｎｕｔｒｉｔｉｏｎａｌ　Ｓｃｉｅｎｃｅ　ａｎｄ　Ｖｉｔａｍｉｎｏｌｏｇｙ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｎｕｔｒｉｔｉｏｎａｌ　Ｓｃｉｅｎｃｅ　ａｎｄ　Ｖｉｔａｍｉｎｏｌｏｇｙ 66 (5), 478-480, 2020-10-31

  一般財団法人 学会誌刊行センター

キーワード

• HSP
• chaperone
• RT-PCR
• acetate
• lactate

詳細情報

• CRID: 1391412326419299456
• NII論文ID: 130007932341
• NII書誌ID: AA00703822
• DOI: 10.3177/jnsv.66.478
• ISSN: 18817742
• ISSN: 03014800
• NDL書誌ID: 030699352
• PubMed: 33132352
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030699352
• Web Site: https://www.jstage.jst.go.jp/article/jnsv/66/5/66_478/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2021233193
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可