Detection of <i>Arcobacter</i> Species in Human Stool Samples by Culture and Real-time PCR

• YAMAUCHI YUKO

  Department of Infectious Diseases, Medical Microbiology and Hygiene, University Hospital Heidelberg Department of Infection Control Science, Juntendo University Graduate School of Medicine

• UEHARA YUKI

  Department of General Medicine, Juntendo University Faculty of Medicine Department of Microbiology, Juntendo University Faculty of Medicine Infection Control Science Research Center, Juntendo University Graduate School of Medicine Department of Clinical Laboratory, St Luke’s International Hospital

• BOUTIN SÉBASTIEN

  Department of Infectious Diseases, Medical Microbiology and Hygiene, University Hospital Heidelberg

• YAMAMOTO NORIO

  Department of Infection Control Science, Juntendo University Graduate School of Medicine Department of Microbiology, Juntendo University Faculty of Medicine

• KUWAHARA-ARAI KYOKO

  Department of Microbiology, Juntendo University Faculty of Medicine

• KIRIKAE TERUO

  Department of Microbiology, Juntendo University Faculty of Medicine Infection Control Science Research Center, Juntendo University Graduate School of Medicine

• HIRAMATSU KEIICHI

  Department of Microbiology, Juntendo University Faculty of Medicine Infection Control Science Research Center, Juntendo University Graduate School of Medicine

• ZIMMERMANN STEFAN

  Department of Infectious Diseases, Medical Microbiology and Hygiene, University Hospital Heidelberg

Bibliographic Information

Other Title

• Detection of Arcobacter Species in Human Stool Samples by Culture and Real-time PCR

Abstract

Objective: The clinical significance of Arcobacter species has not been established due to a lack of suitable detection methods. Material and Methods: A total of 1,650 stool samples submitted to the Clinical Laboratory of Heidelberg University Hospital were inoculated onto agar plates selective for Campylobacter species isolation and incubated at 37℃. Results: Four (0.24%) of the samples were positive for Arcobacter butzleri isolates. Genus-specific primers for real-time PCR were designed to identify Arcobacter species. Of the 1,650 stool samples tested, twelve (0.73%), including the four culture-positive samples, were positive for Arcobacter species by real-time PCR. The sensitivity of real-time PCR was 10 ⁴ CFU g ^-1 stool, 50 CFU reaction ^-1 using a stool sample. Conclusions: Although the sensitivity of real-time PCR was relatively low compared with other PCR methods, the present method detected a broad range of Arcobacter species. The combination of the stool culture using agar selective for Campylobacter species and real-time PCR for Arcobacter species may be clinically useful for the diagnosis and epidemiology of Arcobacter species infections.

Journal

• Juntendo Medical Journal

  Juntendo Medical Journal 66 (5), 431-438, 2020

  The Juntendo Medical Society

Keywords

• <i>Arcobacter</i> species
• human
• stool culture
• real-time PCR

Details

• CRID: 1391130851449685248
• NII Article ID: 130007933296
• NII Book ID: AA1262207X
• DOI: 10.14789/jmj.2020.66.jmj19-oa05
• ISSN: 21882126
• ISSN: 21879737
• NDL BIB ID: 030789217
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030789217
• Web Site: https://www.jstage.jst.go.jp/article/jmj/66/5/66_JMJ19-OA05/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2021296475
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed