Sensitive Method for LC Analysis of Therapeutic Monoclonal Antibodies Using a Centrifugal Filtration Device with Adsorption Suppression Treatment
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- SHIBATA Yosuke
- School of Pharmaceutical Sciences, University of Shizuoka
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- YAMADA Tomohiro
- School of Pharmaceutical Sciences, University of Shizuoka
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- SUGIYAMA Eiji
- School of Pharmaceutical Sciences, University of Shizuoka
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- MIZUNO Hajime
- School of Pharmaceutical Sciences, University of Shizuoka
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- TODOROKI Kenichiro
- School of Pharmaceutical Sciences, University of Shizuoka
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<p>Herein, we report an intact LC–native fluorescence analysis method for therapeutic monoclonal antibodies (mAbs) based on a centrifugal filtration device with adsorption suppression treatment. Coating the centrifugal filtration device with MPC monomer suppressed the non-specific adsorption of mAbs, especially in the low concentration range; trastuzumab could be quantitatively and sensitively analyzed in the 0.2–10 μg/mL range. In this analysis, the average concentration factor over the entire concentration range was approximately 25 times. The other mAbs (bevacizumab, rituximab, nivolumab) also showed good linearity with R2 ≥ 0.996, and the average concentration factors were similar to that obtained for trastuzumab. This method can potentially be used in combination with affinity purification for simple and sensitive bioanalysis.</p>
収録刊行物
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- CHROMATOGRAPHY
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CHROMATOGRAPHY 41 (3), 123-128, 2020-10-20
クロマトグラフィー科学会
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詳細情報 詳細情報について
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- CRID
- 1390849376474153216
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- NII論文ID
- 130007933315
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- NII書誌ID
- AA1137755X
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- ISSN
- 13483315
- 13428284
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- NDL書誌ID
- 030727737
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可
