Rapid differentiation of human dental pulp stem cells to neuron-like cells by high K+ stimulation

• Kogo Yuki

  Department of Biology, Waseda University

• Seto Chiaki

  Department of Biology, Waseda University

• Totani Yuki

  Department of Biology, Waseda University

• Mochizuki Mai

  Department of Life Science Dentistry, The Nippon Dental University Department of Developmental and Regenerative Dentistry, The Nippon Dental University School of Life Dentistry at Tokyo Department of Bioscience and Informatics, Faculty of Science and Technology, Keio University Waseda Research Institute for Science and Engineering, Waseda University

• Nakahara Taka

  Department of Developmental and Regenerative Dentistry, The Nippon Dental University School of Life Dentistry at Tokyo

• Oka Kotaro

  Department of Bioscience and Informatics, Faculty of Science and Technology, Keio University Waseda Research Institute for Science and Engineering, Waseda University Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University

• Yoshioka Tohru

  Waseda Research Institute for Science and Engineering, Waseda University Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University

• Ito Etsuro

  Department of Biology, Waseda University Waseda Research Institute for Science and Engineering, Waseda University Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University

書誌事項

タイトル別名

• Rapid differentiation of human dental pulp stem cells to neuron-like cells by high K⁺ stimulation

抄録

As human-origin cells, human dental pulp stem cells (hDPSCs) are thought to be potentially useful for biological and medical experiments. They are easily obtained from lost primary teeth or extracted wisdom teeth, and they are mesenchymal stem cells that are known to differentiate into osteoblasts, chondrocytes, and adipocytes. Although hDPSCs originate from neural crest cells, it is difficult to induce hDPSCs to differentiate into neuron-like cells. To facilitate their differentiation into neuron-like cells, we evaluated various differentiation conditions. Activation of K⁺ channels is thought to regulate the intracellular Ca²⁺ concentration, allowing for manipulation of the cell cycle to induce the differentiation of hDPSCs. Therefore, in addition to a conventional neural cell differentiation protocol, we activated K⁺ channels in hDPSCs. Immunocyto­chemistry and real-time PCR revealed that applying a combination of 3 stimuli (high K⁺ solution, epigenetic reprogramming solution, and neural differentiation solution) to hDPSCs increased their expression of neuronal markers, such as β3-tubulin, postsynaptic density protein 95, and nestin within 5 days, which led to their rapid differentiation into neuron-like cells. Our findings indicate that epigenetic reprogramming along with cell cycle regulation by stimulation with high K⁺ accelerated the differentiation of hDPSCs into neuron-like cells. Therefore, hDPSCs can be used in various ways as neuron-like cells by manipulating their cell cycle.

収録刊行物

• Biophysics and Physicobiology

  Biophysics and Physicobiology 17 (0), 132-139, 2020

  一般社団法人 日本生物物理学会

キーワード

• dental pulp stem cell
• high K⁺ stimulation
• neural differentiation
• neuron-like cell

詳細情報

• CRID: 1390567901497539072
• NII論文ID: 130007937195
• DOI: 10.2142/biophysico.bsj-2020023
• ISSN: 21894779
• Web Site: https://www.jstage.jst.go.jp/article/biophysico/17/0/17_BSJ-2020023/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2022006352
• 本文言語コード: en
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