Characterization of Colibactin-Producing <i>Escherichia coli</i> Isolated from Japanese Patients with Colorectal Cancer

DOI Web Site Web Site PubMed 5 Citations 34 References
  • Yoshikawa Yuko
    School of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Japan School of Food and Nutritional Sciences, University of Shizuoka, Japan
  • Tsunematsu Yuta
    Department of Pharmaceutical Sciences, University of Shizuoka, Japan
  • Matsuzaki Nobuo
    Department of Pharmaceutical Sciences, University of Shizuoka, Japan
  • Hirayama Yuichiro
    Department of Pharmaceutical Sciences, University of Shizuoka, Japan
  • Higashiguchi Fumi
    Department of Pharmaceutical Sciences, University of Shizuoka, Japan
  • Sato Michio
    Department of Pharmaceutical Sciences, University of Shizuoka, Japan
  • Iwashita Yuji
    Department of Tumor Pathology, Hamamatsu University School of Medicine, Japan
  • Miyoshi Noriyuki
    School of Food and Nutritional Sciences, University of Shizuoka, Japan
  • Mutoh Michihiro
    Epidemiology and Prevention Division, Center for Public Health Sciences, National Cancer Center, Japan Department of Molecular-Targeting Cancer Prevention, Kyoto Prefectural University of Medicine, Japan
  • Ishikawa Hideki
    Department of Molecular-Targeting Cancer Prevention, Kyoto Prefectural University of Medicine, Japan
  • Sugimura Haruhiko
    Department of Tumor Pathology, Hamamatsu University School of Medicine, Japan
  • Wakabayashi Keiji
    Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka, Japan
  • Watanabe Kenji
    Department of Pharmaceutical Sciences, University of Shizuoka, Japan

Bibliographic Information

Other Title
  • Characterization of Colibactin-Producing Escherichia coli Isolated from Japanese Patients with Colorectal Cancer

Search this article

Abstract

<p>We investigated the relationship between colibactin-producing (clb+) Escherichia coli and colorectal adenocarcinoma. In total, 729 E. coli colonies were isolated from tumor and surrounding non-tumor regions in resected specimens from 34 Japanese patients; 450 colonies were from the tumor regions and 279 from the non-tumor regions. clb+ bacteria were found in tumor regions of 11 patients (11/34, 32.4%) and they were also detected in the non-tumor regions of 7 out of these 11 patients (7/34, 20.6%). The prevalence of clb+ isolates was 72.7% (327/450) and 44.1% (123/279) in tumor and non-tumor regions, respectively. All the recovered clb+ isolates belonged to the phylogenetic group B2 and were the most predominant type in tumor regions. Hemolytic (α-hemolysin-positive, hlyA+) and non-hemolytic (α-hemolysin-negative, hlyA-) clb+ isolates were obtained from patient #19; however, the prevalence of hlyA+ clb+ isolates was significantly higher in tumor regions (35/43, 81.4%) than in non-tumor regions (3/19, 15.8%). Moreover, a significantly higher production of N-myristoyl-D-asparagine, a by-product of colibactin biosynthesis, was observed in hlyA+ clb+ isolates than in hlyA- clb+ isolates. Our results suggest that hlyA+ clb+ E. coli may have a selective advantage in colorectal colonization and, consequently, might play a role in carcinogenesis. The presence of hlyA+ clb+ bacteria in healthy individuals is a potential risk marker of colorectal cancer.</p>

Journal

  • Japanese Journal of Infectious Diseases

    Japanese Journal of Infectious Diseases 73 (6), 437-442, 2020-11-30

    National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

Citations (5)*help

See more

References(34)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top