Induction of metallothionein isoforms in cultured bovine aortic endothelial cells exposed to cadmium

• Fujie Tomoya

  Department of Environmental Health, Faculty of Pharmaceutical Sciences, Toho University

• Ozaki Yusuke

  Department of Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science

• Takenaka Fukuta

  Department of Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science

• Nishio Misaki

  Department of Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science

• Hara Takato

  Department of Environmental Health, Faculty of Pharmaceutical Sciences, Toho University

• Fujiwara Yasuyuki

  Department of Environmental Health, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

• Yamamoto Chika

  Department of Environmental Health, Faculty of Pharmaceutical Sciences, Toho University

• Kaji Toshiyuki

  Department of Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science

抄録

Metallothionein (MT) is an inducible protein with cytoprotective activity against heavy metals such as cadmium, zinc, and copper. MT-1 and MT-2 are the isoforms of MT induced by and bind the heavy metals. Bovine aortic endothelial cells contain three types of MT genes, namely, MT-1A, MT-1E, and MT-2A; however, the associated protein expression of these MT isoforms has not been identified. In the present study, the expression of MT subisoform proteins in cells treated with cadmium chloride was identified using a high-performance liquid chromatography-inductively coupled plasma-mass spectrometry system. It was revealed that: (1) transcriptional induction of MT-1A by cadmium was markedly more sensitive than that of MT-1E/2A; (2) MT-1A and MT-2A proteins were the predominant MT subisoforms induced by cadmium; and (3) there might be differentiation in the functions of MT-1 and MT-2 against cadmium cytotoxicity, although the actual roles of the MT isoforms in the cells were not distinct. This is the first study to show the differential induction of isoforms of MT proteins in vascular endothelial cells by cadmium.

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｔｏｘｉｃｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｔｏｘｉｃｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ 45 (12), 801-806, 2020

  一般社団法人　日本毒性学会

キーワード

• Cadmium
• Metallothionein
• Isoform
• Subisoform
• Vascular endothelial cell

詳細情報

• CRID: 1391130851445929856
• NII論文ID: 130007948808
• NII書誌ID: AN00002808
• DOI: 10.2131/jts.45.801
• ISSN: 18803989
• ISSN: 03881350
• NDL書誌ID: 031221011
• PubMed: 33268680
• Web Site: http://id.ndl.go.jp/bib/031221011
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031221011
• Web Site: https://www.jstage.jst.go.jp/article/jts/45/12/45_801/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2021342472
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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