Application of Molecularly Imprinted Polymer-modified Potentiometric Sensor for Quantitative Determination of Histamine in Serum

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  • KONISHI Atsuko
    Department of Analytical Chemistry, Kyoto Pharmaceutical University
  • TAKEGAMI Shigehiko
    Department of Analytical Chemistry, Kyoto Pharmaceutical University
  • IDOSAKI Yoko
    Department of Analytical Chemistry, Kyoto Pharmaceutical University
  • KITADE Tatsuya
    Department of Analytical Chemistry, Kyoto Pharmaceutical University

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Abstract

<p>A molecularly imprinted polymer-modified potentiometric histamine (HIS) sensor was prepared and used for quantitative determination of HIS in bovine serum. The calibration curve using the potential responses measured in 1 × 10−3 mol L−1 phosphate buffer (pH 7.4) showed good linearity in the HIS concentration range of 3 × 10−4 to 1 × 10−2 mol L−1 (r = 0.92), with a detection limit of 1.6 × 10−4 mol L−1. In bovine serum samples, the HIS sensor showed good recovery values of 91 – 104%. Therefore, this HIS sensor successfully determined the HIS concentration in bovine serum samples.</p>

Journal

  • Analytical Sciences

    Analytical Sciences 36 (12), 1561-1563, 2020-12-10

    The Japan Society for Analytical Chemistry

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