Fluorescence Discrimination of Pharmacological Effects on the Na⁺-Ca²⁺ Exchanger and Sarco-Endoplasmic Reticulum Ca²⁺-ATPase in Mouse Ventricular Cardiomyocytes

• Namekata Iyuki

  Department of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University

• Odaka Ryosuke

  Department of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University

• Hamazaki Shunsuke

  Department of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University

• Nisaka Hina

  Department of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University

• Hamaguchi Shogo

  Department of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University

• Tanaka Hikaru

  Department of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University

書誌事項

タイトル別名

• Fluorescence Discrimination of Pharmacological Effects on the Na⁺–Ca²⁺ Exchanger and Sarco-Endoplasmic Reticulum Ca²⁺-ATPase in Mouse Ventricular Cardiomyocytes

抄録

We developed a method to evaluate the activity of the Na⁺–Ca²⁺ exchanger (NCX) and sarco-endoplasmic reticulum Ca²⁺-ATPase (SERCA) with fluorescence microscopy in mouse ventricular cardiomyocytes. In non-beating ventricular cardiomyocytes, α-adrenoceptor stimulation by phenylephrine caused a decrease in the cytoplasmic Ca²⁺ concentration, which was inhibited by SEA0400, an NCX inhibitor, but not cyclopiazonic acid, a SERCA inhibitor. β-Adrenoceptor stimulation by isoprenaline caused a decrease in the cytoplasmic Ca²⁺ concentration, which was inhibited by cyclopiazonic acid but not SEA0400. Ellagic acid, a phenolic phytochemical, also decreased the basal Ca²⁺ concentration, which was inhibited by cyclopiazonic acid, but not SEA0400. Thus, this method using fluorescent microscopy and specific inhibitors would be useful for the evaluation of pharmacological agents acting on NCX and SERCA.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 44 (1), 154-157, 2021-01-01

  公益社団法人 日本薬学会

キーワード

• sarco-endoplasmic reticulum Ca²⁺-ATPase
• Na⁺–Ca²⁺ exchanger
• Ca²⁺ imaging
• ellagic acid

詳細情報

• CRID: 1390005506388415360
• NII論文ID: 130007965347
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.b20-00701
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 031210216
• PubMed: 33132367
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031210216
• Web Site: https://www.jstage.jst.go.jp/article/bpb/44/1/44_b20-00701/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2021325547
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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