Latent 1,3-β-D-glucan acts as an adjuvant for allergen-specific IgE production induced by Japanese cedar pollen exposure

• Kanno Takashi

  Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

• Adachi Yoshiyuki

  Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

• Ohashi-Doi Katsuyo

  Research Laboratory, Torii Pharmaceutical Co., Ltd.

• Matsuhara Hiroki

  Research Laboratory, Torii Pharmaceutical Co., Ltd.

• Hiratsuka Rie

  Division of Biology, Department of Natural Science, The Jikei University School of Medicine

• Ishibashi Ken-ichi

  Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

• Yamanaka Daisuke

  Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

• Ohno Naohito

  Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

抄録

Background: The pollen grains of several plant species contain 1,3-β-D-glucan (BG). BG activates dendritic cells (DCs) and subsequently regulates the innate immune responses. Within Japan, the most common disease associated with type-I hypersensitivity is Japanese cedar pollinosis. However, the role of BG in Japanese cedar pollen (JCP) remains unclear. This study examined the localization and immunological effects of BG in JCP. Methods: The localization of BG in JCP grain was determined by immunohistochemical staining using a soluble dectin-1 protein probe and a BG recognition protein (BGRP). The content of BG extracted from JCP was measured by a BGRP-based ELISA-like assay. The cytokine production by bone marrow-derived DCs (BMDCs) obtained from wild-type and BG receptor (dectin-1) knock-out mice was examined in vitro. The mice were intranasally administered JCP grains and the specific serum Ig levels were then quantified. Results: BG was detected in the exine and cell wall of the generative cell and tube cell of the JCP grain. Moreover, BG in the exine stimulated production of TNF-α and IL-6 in the BMDCs via a dectin-1-dependent mechanism. Meanwhile, JCP-specific IgE and IgG were detected in the serum of wild-type mice that had been intranasally administered with JCP grains. These mice also exhibited significantly enhanced sneezing behavior. However, dectin-1 knock-out mice exhibited significantly lower JCP-specific IgE and IgG levels compared to wild-type mice. Conclusions: Latent BG in JCP can act as an adjuvant to induce JCP-specific antibody production via dectin-1.

収録刊行物

• Allergology International

  Allergology International 70 (1), 105-113, 2021

  一般社団法人日本アレルギー学会

キーワード

• C-type lectin
• Dendritic cells
• Japanese cedar pollen
• Pollen
• Seasonal allergic rhinitis

詳細情報

• CRID: 1390568456351226112
• NII論文ID: 130007966804
• DOI: 10.1016/j.alit.2020.08.004
• ISSN: 14401592
• ISSN: 13238930
• Web Site: https://api.elsevier.com/content/article/PII:S1323893020301076?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S1323893020301076?httpAccept=text/plain
• Web Site: https://search.jamas.or.jp/link/ui/2021283432
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
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