SAXS Signals from Double-Stranded DNA under Shear-Flow Conditions

• Fujita Yosuke

  Chuo University

• Kato-Minoura Takako

  Chuo University

• Alessandro Longo

  ESRF

• Wada Yuko

  Ochanomizu University

• Yagi Toshiki

  Prefectural University of Hiroshima

• Iwamoto Hiroyuki

  SPring-8/JASRI

• Kamiya Ritsu

  Chuo University

• Kamimura Shinji

  Chuo University

Bibliographic Information

Other Title

• 剪断流条件下のDNA二重ラセン溶液で観察されたSAXS信号

Abstract

SAXS signals from the DNA of salmon spermatozoa in an aqueous solution were analyzed using a rheometer-device, which controlled the rate of shearing during SAXS observations. When shearing rate > 1000 s⁻¹ was applied to the DNA suspension, DNA strands were not aligned as observed with other biological filaments, but a stable diffraction peak approximately ranging 1.7–1.8 [nm⁻¹] of Q-values were observed. We compared our observation with simulated SAXS signals.

Journal

• SPring-8/SACLA Research Report

  SPring-8/SACLA Research Report 8 (3), 469-472, 2020-10-29

  Japan Synchrotron Radiation Research Institute

Keywords

• DNA
• solution structure
• scattering
• SAXS simulation
• 2012B1172
• BL45XU

Details

• CRID: 1391131406306243200
• NII Article ID: 130007969743
• DOI: 10.18957/rr.8.3.469
• ISSN: 21876886
• Text Lang: en
• Data Source:
  • JaLC
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed