Ozone Water Bactericidal and Cleaning Effects on Oral Diseases-related Planktonic and Bacterial Biofilms

• Murakami Masahiro

  Department of Gerodontology and Home Care Dentistry, Aichi Gakuin University Department of Microbiology, School of Dentistry, Aichi Gakuin University

• Nagano Keiji

  Division of Microbiology, Department of Oral Biology, School of Dentistry, Health Sciences University of Hokkaido

• Hamaoka Kouichi

  Maxell, Ltd.

• Kato Daisuke

  Department of Gerodontology and Home Care Dentistry, Aichi Gakuin University

• Kawai Tatsushi

  Department of Dental Materials Science, School of Dentistry, Aichi Gakuin University

• Murakami Hiroshi

  Department of Gerodontology and Home Care Dentistry, Aichi Gakuin University

• Hasegawa Yoshiaki

  Department of Microbiology, School of Dentistry, Aichi Gakuin University

抄録

Ozone water has long been known as a bactericidal disinfectant. However, the bactericidal effect of ozone water on bacteria associated with oral diseases has not been thoroughly examined. Further, although oral bacteria reside in biofilms, few studies have explored the effects of ozone water on biofilms. In this study, we aimed to investigate the bactericidal effect of ozone water on bacteria and bacterial biofilms associated with oral diseases. We examined the bactericidal and cleaning effects of ozone water on pathogenic bacteria associated with oral diseases (Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus mutans, and Porphyromonas gingivalis) under planktonic and biofilm growth conditions. When planktonic bacteria were exposed to 5-ppm ozone water, a remarkable antibacterial activity was observed against all of the tested bacterial species. Contrarily, biofilms showed high resistance to ozone water; the bacterial load only slightly decreased even after repeated exposure to ozone water. However, when ozone water was continuously applied at a low flow rate to the biofilms on polystyrene disks, the number of bacteria on the disks was significantly decreased. Our results have shown that the continuous application of ozone water can eliminate oral disease-related bacteria even in biofilms.

収録刊行物

• Journal of Hard Tissue Biology

  Journal of Hard Tissue Biology 30 (1), 27-32, 2021

  硬組織再生生物学会

キーワード

• Ozone water
• Bactericidal
• Planktonic
• Biofilm
• Oral

詳細情報

• CRID: 1391694356262422144
• NII論文ID: 130007975647
• NII論文ID: 40022478775
• NII書誌ID: AA11074332
• DOI: 10.2485/jhtb.30.27
• ISSN: 1880828X
• ISSN: 13417649
• NDL書誌ID: 031273828
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031273828
• Web Site: https://www.jstage.jst.go.jp/article/jhtb/30/1/30_27/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2021325658
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可