Optimal dilutions of S-PRG filler eluate for experiments on human gingival fibroblasts <i>in vitro </i>
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- KASHIWAGI Kousuke
- Department of Fixed Prosthodontics and Occlusion, Osaka Dental University
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- INOUE Hiroshi
- Department of Physiology, Osaka Dental University
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- KOMASA Reiko
- Department of Operative Dentistry, Osaka Dental University
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- HOSOYAMA Yukiko
- Department of Orthodontics, Osaka Dental University
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- YAMASHITA Koki
- Department of Physiology, Osaka Dental University
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- MORISAKI Ayumu
- Department of Physiology, Osaka Dental University
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- GODA Seiji
- Department of Physiology, Osaka Dental University
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抄録
<p>The present study attempted to identify the optimal dilution at which at which the effects of surface reaction-type pre-reacted glass-ionomer (S-PRG) filler eluate on human gingival fibroblasts (HGF) may be safely examined in vitro. S-PRG filler is a material that releases six ions and exerts strong caries-suppressing effects. We prepared S-PRG filler eluate in which S-PRG filler and α-MEM were mixed as a medium for HGF. This eluate contains six ions that are released from S-PRG filler. All cells died in proliferation experiments on HGF using S-PRG filler eluate, which demonstrated that unless S-PRG filler eluate was diluted, the ion concentration was strongly cytotoxic. S-PRG filler eluate diluted by 1/100 or more with the addition of 2% or more of FBS was safe for use. We herein successfully established the optimal dilution of S-PRG filler eluate at which HGF may be safely examined in vitro.</p>
収録刊行物
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- Dental Materials Journal
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Dental Materials Journal 40 (1), 136-142, 2021-01-25
日本歯科理工学会
