書誌事項
- タイトル別名
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- Full automation of sample pretreatment operations by supported liquid extraction for the determination of psychotropic drugs in blood
- 血中向精神薬分析における固相担体カラムを用いた液-液抽出法による前処理の全自動化
- ケッチュウコウ セイシンヤク ブンセキ ニ オケル コソウタンタイ カラム オ モチイタ エキ-エキ チュウシュツホウ ニ ヨル マエショリ ノ ゼン ジドウカ
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抄録
<p> Supported liquid extraction (SLE) is commonly used to avoid emulsion formation; however, it takes a long time to concentrate the eluate and it is difficult to use in multiple samples. To solve these problems, automation of SLE has been previously reported, but no paper has reported the automation of the entire pretreatment operation in one apparatus. Therefore, we applied ATLAS-LEXT, an automated pretreatment apparatus developed to fully automate liquid-liquid extraction (LLE), to SLE and investigated the full automation of the pretreatment operation in a blood drug analysis. Blood samples containing 18 benzodiazepines and 16 other psychotropic drugs were pretreated with ATLAS-LEXT and analyzed using liquid chromatography/mass spectrometry (LC/MS). For all benzodiazepines tested, the calibration curves showed good linearity within the range of 10-500 ng/mL, and the recovery rate was ≥79%. For the other psychotropic drugs, the calibration curve showed good linearity within the range of 10-500 ng/mL, although the recovery rate was low for some analytes. In conclusion, the present study suggests that ATLAS-LEXT is useful for the pretreatment of blood samples for drug analysis using SLE, which has been impossible to fully be automated until now.</p>
収録刊行物
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- 日本法科学技術学会誌
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日本法科学技術学会誌 26 (1), 91-102, 2021
日本法科学技術学会