Analysis of α-Defensin 5 Secretion in Differentiated Caco-2 Cells: Comparison of Cell Bank Origin

• Yasuda Genki

  Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharma Sciences, Faculty of Pharmaceutical Sciences, Hokkaido University

• Kobayashi Masaki

  Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharma Sciences, Faculty of Pharmaceutical Sciences, Hokkaido University

• Kubota Atsuhito

  Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharma Sciences, Faculty of Pharmaceutical Sciences, Hokkaido University

• Narumi Katsuya

  Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharma Sciences, Faculty of Pharmaceutical Sciences, Hokkaido University

• Furugen Ayako

  Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharma Sciences, Faculty of Pharmaceutical Sciences, Hokkaido University

• Saito Yoshitaka

  Department of Pharmacy, Hokkaido University Hospital

• Satoh Takashi

  Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University of Science

• Suzuki Natsuko

  Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University of Science

• Iseki Ken

  Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharma Sciences, Faculty of Pharmaceutical Sciences, Hokkaido University

Abstract

α-Defensin 5 has a particularly broad antibacterial spectrum; it eliminates pathogenic microorganisms and regulates intestinal flora. Although Caco-2 cells are similar to small intestinal cells, it is unclear whether they secrete α-defensin 5. Therefore, we investigated whether Caco-2 cells secrete α-defensin 5 and determined the secretion mechanism using cells from three cell banks (ATCC, DSMZ, and RIKEN). The Caco-2 cell proliferation rate increased with the number of culture days, irrespective of cell bank origin. On the other hand, the alkaline phosphatase activity, which affects cell differentiation and the mRNA levels of several cytokines, such as interleukin 8 (IL-8), IL-6, IL-1β, tumor necrosis factor-α (TNF-α), and IL-2, in the Caco-2 cells fluctuated with the number of culture days, and differed for each cell bank. α-Defensin 5 secretion was detected in all three cell bank Caco-2 cells; particularly, the ATCC Caco-2 cells grew linearly depending on the cell culture day as well as the levels of IL-8 and TNF-α mRNA. This suggested that α-defensin 5 secretion in the ATCC Caco-2 cells was associated with fluctuations in the mRNA levels of various cytokines, such as IL-8 and TNF-α. In conclusion, Caco-2 cells may be a simple model for screening health food components and drugs that affect α-defensin 5 secretion.

Journal

• Biological and Pharmaceutical Bulletin

  Biological and Pharmaceutical Bulletin 44 (2), 275-278, 2021-02-01

  The Pharmaceutical Society of Japan

Keywords

• α-defensin 5
• Caco-2 cell
• cytokine
• differentiation
• alkaline phosphatase

Details

• CRID: 1391131406310892544
• NII Article ID: 130007979605
• NII Book ID: AA10885497
• DOI: 10.1248/bpb.b20-00644
• ISSN: 13475215
• ISSN: 09186158
• NDL BIB ID: 031242494
• PubMed: 33518681
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031242494
• Web Site: https://www.jstage.jst.go.jp/article/bpb/44/2/44_b20-00644/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2022017617
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed