Multiple Hydrogen Loss and Radical-Directed Dissociation of Peptide in MALDI-ISD MS
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- Miyazawa Kei
- Graduate School of Nanobioscience, Yokohama City University
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- Takayama Mitsuo
- Graduate School of Nanobioscience, Yokohama City University
Bibliographic Information
- Other Title
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- MALDI-ISDにおけるペプチドからの複数水素の脱離とラジカル誘導分解
- MALDI-ISD ニ オケル ペプチド カラ ノ フクスウ スイソ ノ ダツリ ト ラジカル ユウドウ ブンカイ
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Abstract
<p>The formation and radical-directed dissociation (RDD) of multiple hydrogen abstracted peptide cations [M+H-mH]•+ have been examined using MALDI In-source decay (ISD) with four different dinitro-substituted matrices, i.e., 3,5-dinitrosalicylic acid (3,5-DNSA), 3,4-dinitrobenzoic acid (3,4-DNBA), 1,5-dinitronaphthalene (1,5-DNN) and 2,4-dinitro-1-naphthol (2,4-DNNL). The MALDI-ISD of peptides using 3,5-DNSA and 3,4-DNBA as matrices resulted in multiple hydrogen abstraction from protonated peptide [M+H]+ and production of fragment [a]+ ions, i.e., [M+H-mH]•+ and [a-mH]•+ (m=1–6). Fragmentation pattern of the intensity of [an]+ ions with 4-nitro-1-naphthol (4,1-NNL) depended on amino acid residue, while that of the [an]+ ions with 3,5-DNSA did not.</p>
Journal
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- Journal of the Mass Spectrometry Society of Japan
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Journal of the Mass Spectrometry Society of Japan 69 (1), 1-3, 2021-02-01
The Mass Spectrometry Society of Japan
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Details 詳細情報について
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- CRID
- 1390005667263237120
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- NII Article ID
- 130007986230
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- NII Book ID
- AN0010555X
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- ISSN
- 18804225
- 18843271
- 13408097
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- NDL BIB ID
- 031293008
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed