MALDI-MS Analysis of Antitumor Antibiotics with Thiol Containing Matrix

• Yoshikawa Hajime

  Graduate School of Nanobioscience, Yokohama City University

• Takayama Mitsuo

  Graduate School of Nanobioscience, Yokohama City University

Bibliographic Information

Other Title

• チオール含有マトリックスを用いた抗腫瘍抗生物質のMALDI-MS分析
• チオール ガンユウ マトリックス オ モチイタ コウシュヨウ コウセイ ブッシツ ノ MALDI-MS ブンセキ

Abstract

The diynene natural products are effective drugs for cancer or leukemia owing to their strong DNA cleavage activity. Bioreduction triggers Masamune–Bergman cyclization of their diynene portion producing a 1,4-dehydrobenzene diradical species, and it abstracts two hydrogens from deoxyribose. MALDI mass spectrum of Esperamicin-A₁, one of the first isolated diynene antibiotics, with thiol containing matrix (2,5-diamino-1,4-benzenedithiol) gave peaks of the cleavage of the trisulfide with thiols (m/z 1248) and cyclized analyte (m/z 1250, Esperamicin-Z).However, three matrices including 2,3-diaminonaphthol gave no peaks of Esperamicin-Z, while it gave peaks of m/z 1248. The results suggested that thiol group of matrix has influence on cyclization mechanism of the diynene portion.

Journal

• Journal of the Mass Spectrometry Society of Japan

  Journal of the Mass Spectrometry Society of Japan 69 (1), 4-7, 2021-02-01

  The Mass Spectrometry Society of Japan

Details

• CRID: 1390287142242655616
• NII Article ID: 130007986233
• NII Book ID: AN0010555X
• DOI: 10.5702/massspec.s21-01
• ISSN: 18804225
• ISSN: 18843271
• ISSN: 13408097
• NDL BIB ID: 031293013
• Web Site: http://id.ndl.go.jp/bib/031293013
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031293013
• Web Site: https://www.jstage.jst.go.jp/article/massspec/69/1/69_S21-01/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed