Functional characterization of testis-brain RNA-binding protein, TB-RBP/Translin, in translational regulation

• OYAMA Kanako

  Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Ibaraki 305-8577, Japan Faculty of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8572, Japan

• BABA Tadashi

  Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Ibaraki 305-8577, Japan Faculty of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8572, Japan Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Ibaraki 305-8577, Japan

• KASHIWABARA Shin-ichi

  Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Ibaraki 305-8577, Japan Faculty of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8572, Japan Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Ibaraki 305-8577, Japan

抄録

Testis-brain RNA-binding protein (TB-RBP/Translin) is known to contribute to the translational repression of a subset of haploid cell-specific mRNAs, including protamine 2 (Prm2) mRNA. Mutant mice lacking TB-RBP display abnormal spermatogenesis, despite normal male fertility. In this study, we carried out functional analysis of TB-RBP in mammalian cultured cells to understand the mechanism of translational repression by this RNA-binding protein. Although the amino acid sequence contained a eukaryotic translation initiation factor 4E (EIF4E)-recognition motif, TB-RBP failed to interact with EIF4E. In cultured cells, TB-RBP was unable to reduce the activity of luciferase encoded by a reporter mRNA carrying the 3’-untranslated region of Prm2. However, λΝ-BoxB tethering assay revealed that the complex of TB-RBP with its binding partner, Translin-associated factor X (TRAX), exhibits the ability to reduce the luciferase reporter activity by degrading the mRNA. These results suggest that TB-RBP may play a regulatory role in determining the sequence specificity of TRAX-catalyzed mRNA degradation.

収録刊行物

• Journal of Reproduction and Development

  Journal of Reproduction and Development 67 (1), 35-42, 2021

  公益社団法人 日本繁殖生物学会

キーワード

• mRNA degradation
• Spermatogenesis
• Testis-brain RNA-binding protein (TB-RBP)/Translin
• Tethering reporter assay
• Translin-associated factor X (TRAX)

詳細情報

• CRID: 1390568617219344384
• NII論文ID: 130007986238
• NII書誌ID: AA10936678
• DOI: 10.1262/jrd.2020-120
• ISSN: 13484400
• ISSN: 09168818
• NDL書誌ID: 031286861
• PubMed: 33268667
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031286861
• Web Site: https://www.jstage.jst.go.jp/article/jrd/67/1/67_2020-120/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2022006437
• 本文言語コード: en
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