Electrolysis of selectively patterned <i>Vorticella</i> with pneumatic microchambers and electrodes
-
- NAGAI Moeto
- Department of Mechanical Engineering, Toyohashi University of Technology
-
- KOHARA Ryoga
- Department of Mechanical Engineering, Toyohashi University of Technology
-
- SANTRA Tuhin Subhra
- Department of Engineering Design, Indian Institute of Technology Madras
-
- SHIBATA Takayuki
- Department of Mechanical Engineering, Toyohashi University of Technology
抄録
<p>Living machines are expected to expand the limits of silicon microtechnology. Vorticella convallaria is a ciliated protozoa and a promising linear actuator. The use of Vorticella as a Ca2+-responsive actuator, in a small system, requires a reduction in the size of the membrane treatment of selectively patterned Vorticella. However, selective cell patterning and the electrolysis of cell membranes have not yet been combined; therefore, the development of a combined method is essential. The objective of this study is to develop a method for combining selective cell patterning and the electrolysis of cell membranes. A two-layer pneumatic device was fabricated in polydimethylsiloxane (PDMS), and the device and electrodes were clamped with acrylic plates. The deformation of the PDMS membrane was controlled by compressed air. The electrochemical reactions were visualized with fluorescein, and the temporal pH change was evaluated. Vorticella cells were introduced into a microfluidic device and were captured in chambers at a pressure of 21 kPa. They were immobilized in the chambers after incubation for 6 h. Further, OH– was generated from the cathode by the electrolysis of water, and the bell-shaped bodies of Vorticella became spherical. This permeabilization treatment stopped both spontaneous contraction and cilia movement. The application of DC 3 V was sufficient for the electrolysis of Vorticella. Selective patterning and electrochemical membrane treatments are beneficial for facilitating the use of Vorticella as a Ca2+ responsive actuator in a small system.</p>
収録刊行物
-
- Mechanical Engineering Journal
-
Mechanical Engineering Journal 8 (1), 20-00254-20-00254, 2021
一般社団法人 日本機械学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390005667266604288
-
- NII論文ID
- 130007986846
-
- ISSN
- 21879745
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可