Molecular detection of filarial nematode parasites in japanese black bears ( Ursus thibetanus japonicus ) from iwate prefecture , japan

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  • MASATANI Tatsunori
    Laboratory of Zoonotic Diseases, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan
  • KOJIMA Isshu
    Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan
  • TASHIRO Michiyo
    Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan
  • YAMAUCHI Kiyoshi
    Laboratory of Wildlife Management, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan
  • FUKUI Daisuke
    Division of Small Animal Surgery, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan
  • ICHIKAWA-SEKI Madoka
    Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan
  • HARASAWA Ryo
    Iwate Research Center for Wildlife Disease, 1-17-4 Nakano, Morioka, Iwate 020-0816, Japan

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タイトル別名
  • Molecular detection of filarial nematode parasites in Japanese black bears (<i>Ursus thibetanus japonicus</i>) from Iwate Prefecture, Japan

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抄録

<p>This study aimed to detect filarial parasites in blood samples of Japanese black bears (Ursus thibetanus japonicus) collected from Iwate Prefecture, Japan. Positive amplicons were obtained from 26 out of 30 samples by nested PCR targeting 18S ribosomal RNA gene and first internal transcribed spacer regions. DNA sequences of Mansonella sp. close to M. ozzardi and Dirofilaria sp. were detected for eight and 11 positive amplicons, respectively. Co-infection was detected for the remaining seven amplicons. Dirofilaria sp. was identified as D. ursi by further genetic analysis of 5S ribosomal RNA gene sequence. The results of this study will contribute to further investigations of Japanese black bears for monitoring their risk as a reservoir of possible zoonotic filarial parasites.</p>

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