Roles of Layilin in Regulation of Low-Density Lipoprotein Receptor in Malignant Glioma Cells

• Ushimaru Shu

  Clinical Proteomics and Molecular Medicine, St. Marianna University Graduate School of Medicine Division of Nephrology and Hypertension, Department of Internal Medicine, St. Marianna University School of Medicine

• Arito Mitsumi

  Clinical Proteomics and Molecular Medicine, St. Marianna University Graduate School of Medicine

• Tsutiya Atsuhiro

  Clinical Proteomics and Molecular Medicine, St. Marianna University Graduate School of Medicine

• Sato Toshiyuki

  Clinical Proteomics and Molecular Medicine, St. Marianna University Graduate School of Medicine

• Omoteyama Kazuki

  Clinical Proteomics and Molecular Medicine, St. Marianna University Graduate School of Medicine

• Sato Masaaki

  Clinical Proteomics and Molecular Medicine, St. Marianna University Graduate School of Medicine

• Suematsu Naoya

  Clinical Proteomics and Molecular Medicine, St. Marianna University Graduate School of Medicine

• S. Kurokawa Manae

  Disease Biomarker Analysis and Molecular Regulation, St. Marianna University Graduate School of Medicine

• Kamijo-Ikemori Atsuko

  Division of Nephrology and Hypertension, Department of Internal Medicine, St. Marianna University School of Medicine

• Shibagaki Yugo

  Division of Nephrology and Hypertension, Department of Internal Medicine, St. Marianna University School of Medicine

• Kato Tomohiro

  Clinical Proteomics and Molecular Medicine, St. Marianna University Graduate School of Medicine

抄録

Objective: Layilin is a type I transmembrane protein that possesses a C-type lectin motif in its extracellular domain, and whose functions have not yet been completely understood. Previously, we reported that layilin was highly expressed in malignant glioma cells. In this study, we investigated whether layilin was functionally related to low-density lipoprotein receptor (LDLR) in malignant glioma cells because one of the characteristics of malignant glioma cells is the high expression of LDLR.<br/>Methods: Under layilin-knockdown (KD) conditions in A172 cells, a malignant glioma cell line, we measured LDLR mRNA and protein levels by quantitative polymerase chain reaction and western blotting, respectively. Furthermore, we measured LDL uptake in layilin-KD cells by LDL uptake assay.<br/>Results: Even though the amounts of mRNA for LDLR were unaffected by layilin-KD, the amounts of LDLR protein were significantly increased by layilin-KD 48 h after transfection with small interfering RNAs for layilin (p<0.05). Accordingly, LDL uptake was increased by layilin-KD (p<0.05).<br/>Conclusion: Our data suggest a novel function of layilin, that is, down-regulation of LDLR at the protein level.

収録刊行物

• Journal of St. Marianna University

  Journal of St. Marianna University 11 (2), 53-59, 2020

  学校法人 聖マリアンナ医科大学医学会

キーワード

• Layilin
• LDL
• LDL receptor

詳細情報

• CRID: 1390287297543697280
• NII論文ID: 130007992048
• DOI: 10.17264/stmarieng.11.53
• ISSN: 21890277
• ISSN: 21851336
• Web Site: https://www.jstage.jst.go.jp/article/stmarieng/11/2/11_53/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2022017805
• 本文言語コード: en
• データソース種別:
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