Application of Conventional FE-SEM to Podocyte Structural Analysis: Protocol and Usage Examples

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  • KAWASAKI YUTO
    Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine
  • MIYAKI TAKAYUKI
    Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine
  • YAMAGUCHI JUNJI
    Laboratory of Morphology and Image Analysis, Research Support Center, Juntendo University Graduate School of Medicine
  • KAKUTA SOICHIRO
    Laboratory of Morphology and Image Analysis, Research Support Center, Juntendo University Graduate School of Medicine
  • HOSOYAMADA YASUE
    Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine
  • SAKAI TATSUO
    Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine
  • ICHIMURA KOICHIRO
    Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine Laboratory of Morphology and Image Analysis, Research Support Center, Juntendo University Graduate School of Medicine

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Abstract

<p>　Podocytes, an epithelial cell type specialized for glomerular filtration, exhibit a complex projection structure. Field-emission scanning electron microscopy (FE-SEM) is a powerful approach to observing the surface structure of podocytes, particularly their pathologic alterations, including foot process effacement. Here, we introduce the conventional FE-SEM protocol for three-dimensional visualization of podocyte surface structure in our institution. This method is reproducible and allows us to visualize the luminal surface of numerous podocytes simultaneously.</p>

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