Separation of Dansyl-DL-Amino Acids Through Tube Radial Distribution Chromatography by Using a Commercially Available HPLC System with a Capillary Tube Manufactured for GC as a Separation Column

• SANADA Nobuyuki

  Applied Chemistry, Graduate School of Science and Engineering, Doshisha University

• TSUKAGOSHI Kazuhiko

  Applied Chemistry, Graduate School of Science and Engineering, Doshisha University

• TSUCHIYA Katsumi

  Applied Chemistry, Graduate School of Science and Engineering, Doshisha University

• HIROTA Ken

  Applied Chemistry, Graduate School of Science and Engineering, Doshisha University

• YAMASHITA Kenichi

  Advanced Manufacturing Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

• MURATA Masaharu

  Department of Advanced Medical Initiatives, Faculty of Medical Sciences, Kyushu University

抄録

Dansyl-DL-amino acids were separated by tube radial distribution chromatography (TRDC) comprising a commercially available HPLC system, where open-tubular capillary tubes manufactured for capillary GC were used as the separation column. Initially, the separation performance of GC capillary tubes in TRDC was assessed by separating a model analyte mix of 1-naphthol and 2,6-naphthalenedisulfonic acid by using water/acetonitrile/ethyl acetate solutions (3:8:4 volume ratio, organic solvent-rich and 4:3:1 volume ratio, water-rich) as the eluent and high-polarity and non-polarity capillary tubes. Next, enantiomer separation of dansyl-DL-methionine and dansyl-DL-valine was examined using a water/acetonitrile/ethyl acetate solution (8:2:1 volume ratio, water-rich) containing 1.0 mM -cyclodextrin and non-polarity capillary tubing. The D- and L-enantiomers were separated and detected in this order through interaction between amino acids and -cyclodextrin by tube radial distribution flow in the TRDC system.

収録刊行物

• CHROMATOGRAPHY

  CHROMATOGRAPHY 42 (1), 67-71, 2021-02-20

  クロマトグラフィー科学会

キーワード

• Phase separation multiphase flow
• Tube radial distribution chromatography
• HPLC system
• Enantiomer separation
• Dansyl amino acids

詳細情報

• CRID: 1390568772524295424
• NII論文ID: 130007993889
• NII書誌ID: AA1137755X
• DOI: 10.15583/jpchrom.2020.019
• ISSN: 13483315
• ISSN: 13428284
• NDL書誌ID: 031325142
• Web Site: http://id.ndl.go.jp/bib/031325142
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031325142
• Web Site: https://www.jstage.jst.go.jp/article/jpchrom/42/1/42_2020.019/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可