Effect of Organic Modifier on the Retention of Low-Molecular-Weight Organic Compounds in Low-Temperature HPLC Using a Liquid CO<sub>2</sub> Mobile Phase and an Octadecyl Stationary Phase

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  • MATSUO Kohei
    Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology
  • OTSUBO Mina
    Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology
  • MOTONO Tomohiro
    Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology
  • KITAGAWA Shinya
    Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology
  • IIGUNI Yoshinori
    Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology
  • OHTANI Hajime
    Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology

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  • Effect of Organic Modifier on the Retention of Low-Molecular-Weight Organic Compounds in Low-Temperature HPLC Using a Liquid CO₂ Mobile Phase and an Octadecyl Stationary Phase

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Abstract

<p>The retention behaviors of various low-molecular-weight compounds in low-temperature HPLC with an ODS (octadecyl silica) column and liquid CO2 mobile phase containing methanol or acetonitrile as modifiers were investigated. The addition of methanol/acetonitrile reduced the retention of xanthone, which was dominated by the liquid CO2 content, independent of the modifier. Additionally, the retention of polycyclic aromatic hydrocarbons (PAHs) was reduced by the addition of methanol/acetonitrile. Because acetonitrile resulted in a rapid reduction in the retention compared with methanol, it was suggested that the CH- interactions have an important role in the retention of PAHs in the C18 stationary phase. Notably, the retention of alkylbenzenes were only slightly affected by the addition of methanol/acetonitrile. Acetonitrile slightly increased the retention of alkylbenzenes with longer alkyl chains than those with shorter alkyl chains. It is suggested that the interaction of the phenyl moiety in alkylbenzenes with C18 chains was reduced by the increase in the acetonitrile content, and enhanced in the case of the alkyl chain moieties. The retentions of alkyl benzenes were approximately compensated, when the alkyl chain length was relatively short. </p>

Journal

  • CHROMATOGRAPHY

    CHROMATOGRAPHY 42 (1), 61-66, 2021-02-20

    The Society for Chromatographic Sciences

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