Length Discrimination of Homo-oligomeric Nucleic Acids with Single-molecule Measurement

• KOMOTO Yuki

  The Institute of Scientific and Industrial Research, Osaka University Artificial Intelligence Research Center, The Institute of Scientific and Industrial Research, Osaka University

• OHSHIRO Takahito

  The Institute of Scientific and Industrial Research, Osaka University

• TANIGUCHI Masateru

  The Institute of Scientific and Industrial Research, Osaka University

抄録

Single-molecule DNA/RNA sequencing based on single-molecule measurement is a prominent method for higher throughput sequencing. In a previous report, the single-molecule DNA/RNA sequencing method has applied to detect each base-conductance difference in the tunneling current time profiles, and determined the sequence. However, discrimination of identical base lengths has not yet been achieved. The number of the identical contiguous bases has importance in biology because some homopolymers of nucleic acid control gene expression. In this study, we aimed to develop a method for discriminating the length of homopolymer of nucleic acids of adenosine monophosphate (AMP) using a single-molecule sequencing technique. We carried out single-molecule conductance measurements of adenine pentamer, hexamer and heptamer. The single-molecule signals of the oligomers are not distinguishable from current and duration time histograms. The three oligomers were discriminated by our developed machine learning-based analysis with accuracy of 0.54 for a single signal, and 99% for 40 signals. This method will be applied to the single signals and identify the contiguous bases in the sequence and provide new biological insights.

収録刊行物

• Analytical Sciences

  Analytical Sciences 37 (3), 513-518, 2021-03-10

  社団法人 日本分析化学会

キーワード

• DNA
• single-molecule detection
• machine learning
• polyadenine

詳細情報

• CRID: 1390568772521001472
• NII論文ID: 130007996240
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.20scp13
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 031326394
• PubMed: 33310994
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031326394
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.20SCP13.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.20SCP13/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/37/3/37_20SCP13/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2022057690
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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