Strategies for Making Multimeric and Polymeric Bifunctional Protein Conjugates and Their Applications as Bioanalytical Tools

• PERMANA Dani

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University Research Unit for Clean Technology, Indonesian Institute of Sciences (LIPI), Kampus LIPI Bandung

• MINAMIHATA Kosuke

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• GOTO Masahiro

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University Division of Biotechnology, Center for Future Chemistry, Kyushu University

• KAMIYA Noriho

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University Division of Biotechnology, Center for Future Chemistry, Kyushu University

Abstract

Enzymes play a central role in the detection of target molecules in biotechnological fields. Most probes used in detection are bifunctional proteins comprising enzymes and binding proteins conjugated by chemical reactions. To create a highly sensitive detection probe, it is essential to increase the enzyme-to-binding protein ratio in the probe. However, if the chemical reactions required to prepare the probe are insufficiently site-specific, the detection probe may lose functionality. Genetic modifications and enzyme-mediated post-translational modifications (PTMs) can ensure the site-specific conjugation of proteins. They are therefore promising strategies for the production of detection probes with high enzyme contents, i.e., polymeric bifunctional proteins. Herein, we review recent advances in the preparation of bifunctional protein conjugates and polymeric bifunctional protein conjugates for detection. We have summarized research on genetically fused proteins and enzymatically prepared polymeric bifunctional proteins, and will discuss the potential use of protein polymers in various detection applications.

Journal

• Analytical Sciences

  Analytical Sciences 37 (3), 425-437, 2021-03-10

  The Japan Society for Analytical Chemistry

Keywords

• Detection probes
• bifunctional proteins
• site-specific conjugation
• polymeric bifunctional proteins
• protein polymers

Details

• CRID: 1390287297545585664
• NII Article ID: 130007996253
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.20scr07
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 031326187
• PubMed: 33455962
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031326187
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.20SCR07.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.20SCR07/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/37/3/37_20SCR07/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2022057679
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed