Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases

• Katoh Toshihiko

  Graduate School of Biostudies, Kyoto University

• Yamamoto Kenji

  Center for Innovative and Joint Research, Wakayama University

Abstract

Most functional biopharmaceuticals such as antibodies are glycoproteins carrying N-linked oligosaccharides (N-glycans). In animal cells, these glycans are generally expressed as heterogeneous glycoforms that are difficult to separate into a pure form. The structure of these glycans directly affects several biological aspects of the glycoproteins, especially binding affinity. Therefore, the preparation of glycoproteins with well-defined and homogeneous glycoforms is necessary for functional studies and improved efficacy, particularly for biopharmaceuticals. This review describes the recent remarkable progress in the development and production of biopharmaceutical glycan-modified antibodies, through the use of glycan remodeling using microbial endoglycosidases and sophisticated glycoengineering techniques utilizing microbial enzymatic reaction mechanisms.

Journal

• Journal of Applied Glycoscience

  Journal of Applied Glycoscience 68 (1), 1-9, 2021-03-11

  The Japanese Society of Applied Glycoscience

Keywords

• immunoglobulin G
• endo-β-<i>N-</i>acetylglucosaminidase
• transglycosylation
• remodeling
• glycoengineering

Details

• CRID: 1390005888481587968
• NII Article ID: 130007996786
• DOI: 10.5458/jag.jag.jag-2020_0013
• ISSN: 18807291
• ISSN: 13447882
• HANDLE: 2433/274028
• NDL BIB ID: 031345528
• Web Site: http://id.ndl.go.jp/bib/031345528
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031345528
• Web Site: https://www.jstage.jst.go.jp/article/jag/68/1/68_jag.JAG-2020_0013/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2021250384
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed