Preliminary study on local medical treatment of aortic valve calcification: Drug delivery to aortic valve in rat

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  • Yu Zaiqiang
    Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine
  • Liu Xu
    Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine
  • Daitoku Kazuyuki
    Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine
  • Furukawa Ken-Ichi
    Department of Orthopaedic Surgery, Hirosaki University Graduate School of Medicine
  • Fukuda Ikuo
    Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine
  • Motomura Shigeru
    Department of Pharmacology, Hirosaki University Graduate School of Medicine
  • Seya Kazuhiko
    Department of Vascular Biology, Hirosaki University Graduate School of Medicine

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Background<br> Aortic valve stenosis （AVS） is the most common heart valve disease in elderly society. The effective treatment for AVS is surgical aortic valve replacement but with extremely invasive. Although transcatheter aortic valve replacement also is performed for highly risk patients with minimally invasive, it has to resolve the problem of bioprosthetic valve durability in future. However, there is no effective medical treatment established for AVS to inhibit acceleration of aortic valve calcification.<br> Objectives<br> We aimed to confirm whether the ascending aorta is the best location for AVS to innovate local and selective medical therapy or not.<br> Methods and Results<br> After anesthetized by isoflurane, the wild type rat was stablished on operating table. Opening chest to heart by median sternotomy approach, the catheter was inserted into left ventricular from apical for body perfusion. We injected saturated solution of amido black 10B （AB） into ascending aorta above sino-tubular junction about 0.5 cm from aortic valves without aortic dissection observed, and body perfusion was performed by saline containing heparin in order to prevent embolism happened in vascular. After 30 minutes, the heart with the ascending aorta was extracted. We used optical microscope to check the existence of AB at around of aortic valves or not. Interestingly, AB was detected at around of aortic valves and valsalva sinus of the aorta and myocardium in all rats.<br> Conclusions<br> These results suggested that AB injected is delivered from the ascending aorta to aortic valves through vasa vasorum. These data provide very important evidences for local medical therapy development for AVS patients.

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  • 弘前医学

    弘前医学 71 (2-4), 101-107, 2021

    弘前大学大学院医学研究科・弘前医学会

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