Carbonyl reductase 1-overexpressing exosomes inhibit proliferation of ovarian cancer cells Carbonyl reductase 1-overexpressing exosomes inhibit proliferation of ovarian cancer cells

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Author(s)

Abstract

    We previously reported that a human carbonyl reductase 1 (CBR1) DNA-dendrimer complex couldpotentially be used in gene therapy for peritoneal metastasis of ovarian cancer. The aims of the current studywere to make exosomes which overexpressed CBR1 and to examine the antiproliferative effect of using the CBR1-overexpressing exosomes on ovarian cancer cells. Endometrial stromal cells (fibroblasts) were transfected withCBR1 DNA by using Lipofectamine, the highest expression level of CBR1 was produced from the cells transfectedunder the condition of Lipofectamin 24 μl/DNA 36 μg for 48 h. Exosomes were purified from culture supernatants byexoEasy Maxi Kit. Western blot showed that CBR1 notably expressed in exosomes extracted from the stromal cellstransfected with CBR1 DNA. Proliferation of ovarian cancer cell line was significantly inhibited by adding CBR1-overexpressing exosomes compared to proliferation of those cells in which exosomes without CBR1 DNA were added.We obtained the evidence that CBR1-overexpressing exosomes could function in carrying CBR1 DNA into ovariancancer cells. Results suggested that exosomes are a useful tool of gene delivery and that a gene therapy of combiningCBR1 DNA and exosomes may be promoted in the treatment of advanced and recurrent ovarian cancers withperitoneal dissemination.

Journal

  • Hirosaki Medical Journal

    Hirosaki Medical Journal 71(2-4), 120-130, 2021

    Hirosaki University Graduate School of Medicine,Hirosaki Medical Society

Codes

  • NII Article ID (NAID)
    130007998259
  • NII NACSIS-CAT ID (NCID)
    AN00211444
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    0439-1721
  • NDL Article ID
    031337838
  • NDL Call No.
    Z19-71
  • Data Source
    NDL  IR  J-STAGE 
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