<b>Identification, characterization and expression of <i>A</i>-mating type genes in monokaryons and dikaryons of the edible mushroom <i>Mycoleptodonoides aitchisonii</i> (Bunaharitake)</b>

• Riffiani Rini

  The United Graduate School of Agricultural Sciences, Tottori University Research Center of Biology, Indonesia Institute of Science (LIPI)

• Chen Fu-Chia

  Faculty of Agriculture, Tottori University

• Zhang Weitong

  The United Graduate School of Agricultural Sciences, Tottori University

• Wada Takayuki

  Laboratory, Biological Business Department, Ichimasa Kamaboko Co., Ltd.

• Shimomura Norihiro

  Faculty of Agriculture, Tottori University

• Yamaguchi Takeshi

  Faculty of Agriculture, Tottori University

• Aimi Tadanori

  Faculty of Agriculture, Tottori University

Bibliographic Information

Other Title

• Identification, characterization and expression of A-mating type genes in monokaryons and dikaryons of the edible mushroom Mycoleptodonoides aitchisonii (Bunaharitake)

Abstract

Identifying the mating-type in Mycoleptodonoides aitchisonii is important for enhancing breeding and cultivation of this edible mushroom. To clarify the molecular mechanisms of the bipolar mating system in M. aitchisonii, the homeodomain protein gene 2 (Mahd2) was characterized. A genomic DNA fragment of Mahd2 in M. aitchisonii 50005-18 strain was 1,851 bp long and encoded a protein of 614 amino acids. Transcriptional analysis revealed that the expression of Mahd2 was higher in monokaryotic strains that produced clamp cells than in those that did not. The highest relative expression level of Mahd2 was observed in monokaryon TUFC 50005-4, which was capable of forming a true clamp. These results suggested that the formation of clamp cells is regulated by A-mating type homeodomain proteins, and the frequency of clamp cell formation might be promoted by high expression of the Mahd2 gene.

Journal

• Mycoscience

  Mycoscience 62 (2), 106-114, 2021-03-20

  The Mycological Society of Japan

Keywords

• bipolar
• clamp cell
• gene expression
• homeodomain protein
• <i>Mahd2</i>

Details

• CRID: 1390005966210012928
• NII Article ID: 130008000688
• NII Book ID: AA11003436
• DOI: 10.47371/mycosci.2020.11.008
• ISSN: 16182545
• ISSN: 13403540
• NDL BIB ID: 032158417
• Web Site: http://id.ndl.go.jp/bib/032158417
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I032158417
• Web Site: https://www.jstage.jst.go.jp/article/mycosci/62/2/62_MYC521/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Allowed