Intravital Ca<sup>2+ </sup>imagingと遺伝子解析による唾液腺における代償性機能亢進の分子機構の解明

DOI Web Site

書誌事項

タイトル別名
  • Analysis of the molecular mechanism of compensatory hypertrophy-associated enhancement of salivary secretion using the intravital Ca<sup>2+ </sup>imaging and gene analysis.

抄録

<p>Dysfunction of unilateral salivary gland causes compensatoryhypertrophy of the contralateral gland. We developed a method for the intravital Ca2+ imaging with simultaneous monitoring of salivary secretion, and found that ligation of main excretory duct (MED) of unilateral submandibular gland (SMG) induced compensatory hypertrophy in association with enhancement of salivary secretion and Ca2+ response. To clarify the molecular mechanism of this compensatory hyperfunction of SMG, we examined effects of the MED ligation on changes in salivary secretion, Ca2+ response and gene expression in SMG. Salivary secretion and Ca2+ response by low doses of acetylcholine in contralateral SMG were higher than those in control SMG at 21 days after the ligation (21d). Comprehensive analyses of mRNA expression and qRT-PCR indicate changes in expression of 6 genes at 7 days after the ligation (7d) and 21d. Atropine failed to change the expression of 3 out of 4 marker genes at 7d, suggesting the involvement of non-cholinergic mechanisms. We also found the increase in expression of amylase, an acinar cell marker gene, and the number of PCNA-positive cells in SMG acinar cells at 3 days after the ligation. These results suggest the compensatory hyperfunction of SMG is associated with the enhanced proliferation of acinar cells.</p>

収録刊行物

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ