Growth Characteristics of <i>Rickettsia</i> Species LON Strains Closely Related to <i>Rickettsia</i> <i>japonica</i> Isolated from <i>Haemaphysalis longicornis</i> Ticks in Mouse Derived L929 and Human-Derived THP-1 Host Cell Lines

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  • Tai Hitoshi
    Graduate Program in Pharmaceutical and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, Japan
  • Su Hongru
    Graduate Program in Pharmaceutical and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, Japan
  • Takamoto Naoya
    Graduate Program in Pharmaceutical and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, Japan
  • Fujita Hiromi
    Graduate Program in Pharmaceutical and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, Japan Mahara Institute of Medical Acarology, Japan
  • Takano Ai
    Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Yamaguchi University, Japan
  • Oishi Saori
    Department of Microbiology, Shizuoka Institute of Environment and Hygiene, Japan
  • Abe Fuyuki
    Department of Microbiology, Shizuoka Institute of Environment and Hygiene, Japan
  • Ando Shuji
    Department of Virology I, National Institute of Infectious Diseases, Japan
  • Ohashi Norio
    Graduate Program in Pharmaceutical and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, Japan

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Other Title
  • Growth Characteristics of Rickettsia Species LON Strains Closely Related to Rickettsia japonica Isolated from Haemaphysalis longicornis Ticks in Mouse-Derived L929 and Human-Derived THP-1 Host Cell Lines

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Abstract

<p>Non-pathogenic Rickettsia species LON strains closely related to an agent of Japanese spotted fever (JSF), R. japonica, were isolated in Japan from Haemaphysalis longicornis ticks in 2001. However, the biological properties of LONs in mammalian host cells are poorly understood. In this study, microscopic analysis showed that LONs in a mouse-derived L929 host cell line were rod shaped with sizes of 0.3–0.5 × 0.5–2.0 μm. Molecular analysis revealed the existence of a LON-specific disrupted open reading frame in R. japonica-related group-specific DNA regions. Growth kinetics of LON-2 and LON-13 strains analyzed by a quantitative real-time PCR showed 100-fold or more increment of LONs cultured in L929 host cells at 30°C and slightly less increment at 33°C, and 25-fold increment in human-derived THP-1 host cells at 35°C on day 7 (168 h) post infection. The generation times of the two LON strains cultured in L929 and THP-1 were estimated to be 9.4–12.9 h and 9.6–10.9 h, respectively. To our knowledge, this is the first report on the biological characteristics of Rickettsia sp. LON strains in mammalian cells, which may provide significant information for the experimental approaches for other rickettsiae.</p>

Journal

  • Japanese Journal of Infectious Diseases

    Japanese Journal of Infectious Diseases 74 (2), 102-109, 2021-03-31

    National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

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