Spatio-temporal gene expression analysis from cryosection using laser microdissection

• Masashi Asahina

  Department of Biosciences, Teikyo University Advanced Instrumental Analysis Center, Teikyo University

• Nakanowatari Miyuki

  Department of Biosciences, Teikyo University

• Yamada Kazuki

  Department of Biosciences, Teikyo University

• Yumoto Emi

  Advanced Instrumental Analysis Center, Teikyo University

• Satoh Shinobu

  Faculty of Life and Environmental Sciences, University of Tsukuba

Bibliographic Information

Other Title

• レーザーマイクロダイセクション法を用いた凍結切片からの遺伝子発現解析
• レーザーマイクロダイセクションホウ オ モチイタ トウケツ セッペン カラ ノ イデンシ ハツゲン カイセキ
• 共同プロジェクト研究(実用化開発) 尿より蒸散するホルムアルデヒドを検知する分析チップの画像解析についての研究
• キョウドウ プロジェクト ケンキュウ(ジツヨウカ カイハツ) ニョウ ヨリ ジョウサン スル ホルムアルデヒド オ ケンチ スル ブンセキ チップ ノ ガゾウ カイセキ ニ ツイテ ノ ケンキュウ

Abstract

Laser microdissection (LMD), also called as Laser capture microdissection (LCM), is a method for isolating different tissue cells or specific single cells from wide variety of tissue samples under direct microscopic observation. LMD method enables to harvest the cells of interest region or specific cells for several analysis such as DNA/RNA analysis, proteomics, metabolomics and other molecular analysis. Currently, this approach can be used to study various biological events at tissue- or cellular-level, LMD has been used in the wide field of research. In this report, we describe techniques for isolation different tissues/specific cells from cryosections of Arabidopsis incised flowering stem by LMD for spatio-temporal gene expression analyses.

Journal

• PLANT MORPHOLOGY

  PLANT MORPHOLOGY 32 (1), 39-43, 2020

  The Japanese Society of Plant Morphology

Keywords

• <i>Arabidopsis thaliana</i>
• cryosection
• gene expression analysis
• laser microdissection
• spatio-temporal analysis

Details

• CRID: 1390006050800911744
• NII Article ID: 120007168745
• NII Article ID: 130008006486
• NII Book ID: AA11315461
• ISSN: 09152024
• ISSN: 18844154
• ISSN: 09189726
• DOI: 10.5685/plmorphol.32.39
• NDL BIB ID: 031740797
• NDL BIB ID: 030243037
• Web Site: https://tohtech.repo.nii.ac.jp/records/192
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031740797
• Web Site: http://id.ndl.go.jp/bib/030243037
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030243037
• Web Site: https://www.jstage.jst.go.jp/article/plmorphol/32/1/32_39/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed