Tracking the subcellular protein localization process by combining the time-gated method and photoconvertible fluorescence protein

DOI Web Site Web Site 18 References
  • Sakata Momoko
    Center for Bioscience Research and Education, Utsunomiya University United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology
  • Kodama Yutaka
    Center for Bioscience Research and Education, Utsunomiya University United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology

Bibliographic Information

Other Title
  • タイムゲート法と光変換型蛍光タンパク質とを組み合わせた細胞内タンパク質局在過程の追跡
  • タイムゲートホウ ト ヒカリ ヘンカンガタ ケイコウ タンパクシツ ト オ クミアワセタ サイボウ ナイ タンパクシツキョクザイカテイ ノ ツイセキ

Search this article

Abstract

<p>Chlorophyll within chloroplast emits high-intensity autofluorescence, which interferes with the observation of exogenous fluorescent substance such as fluorescent protein in the plant cell. We previously reported that clear fluorescence observation can be done by using the time-gated method that eliminates the autofluorescence. Recently, we succeeded in tracking the subcellular localization process of the blue-light photoreceptor phototropin (Phot) to the chloroplast periphery in the liverwort Marchantia polymorpha by a combination of the confocal laser microscopy for the time-gated method and a photoconvertible fluorescent protein. Here, as an example of the time-gated fluorescence imaging, we introduce a study about intracellular localization and movement of Phot in M. polymorpha.</p>

Journal

  • PLANT MORPHOLOGY

    PLANT MORPHOLOGY 32 (1), 27-30, 2020

    The Japanese Society of Plant Morphology

References(18)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top