Anti-inflammatory Effects of Sanhuang-Siwu-Tang in Lipopolysaccharide-Stimulated RAW264.7 Macrophages and BV2 Microglial Cells

• Li Jing

  Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University Department of Pharmacy, Jiujiang University Affiliated Hospital

• Kim Kwan-Woo

  Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University

• Oh Hyuncheol

  Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University

• Kim Youn-Chul

  Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University

抄録

Sanhuang-Siwu-Tang (SST), composed of seven medicinal herbs, is a well-known herbal formula used for the treatment of gynecologic diseases. To expand the clinical use of SST, we explored the anti-inflammatory or anti-neuroinflammatory effects of SST water extract in lipopolysaccharide-stimulated RAW264.7 macrophages and BV2 microglial cells. According to HPLC analysis, the main components of SST were from Scutellariae Radix, Coptidis Rhizoma, and Paeoniae Radix. SST significantly inhibited pro-inflammatory mediators including lipopolysaccharide (LPS)-induced production of nitric oxide (NO) and prostaglandin E₂ (PGE₂) as well as protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and the production of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in LPS-stimulated RAW264.7 macrophages and BV2 microglial cells. Furthermore, these anti-inflammatory or anti-neuroinflammatory effects of SST were mediated by mitogen-activated protein kinase-related proteins (MAPK) and nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB)-related proteins. Overall, this study demonstrated that SST is a potential therapeutic formula for the prevention or treatment of inappropriate inflammation, neuroinflammation, or neurodegenerative diseases.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 44 (4), 535-543, 2021-04-01

  公益社団法人 日本薬学会

キーワード

• Sanhuang-Siwu-Tang
• inflammation
• neuroinflammation
• RAW264.7 macrophage
• BV2 microglial cell

詳細情報

• CRID: 1390006065650579200
• NII論文ID: 130008014270
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.b20-00871
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 031366566
• PubMed: 33563883
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031366566
• Web Site: https://www.jstage.jst.go.jp/article/bpb/44/4/44_b20-00871/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2022048864
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可