Development of a Single-step Bioassay Microdevice Using a Reagent Immobilization Method Based on Inkjet Printing

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  • KAWAI Yuko
    Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University
  • SHIRAI Akihiro
    Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University
  • KAKUTA Masaya
    Sysmex Corporation
  • IDEGAMI Kotaro
    Sysmex Corporation
  • SUEYOSHI Kenji
    Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University Japan Science and Technology Agency (JST), Precursory Research for Embryonic Science and Technology (PRESTO)
  • ENDO Tatsuro
    Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University
  • HISAMOTO Hideaki
    Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University

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Other Title
  • インクジェットプリンティングによる試薬固定化法を利用する1ステップバイオアッセイマイクロデバイスの開発
  • インクジェットプリンティング ニ ヨル シヤク コテイカホウ オ リヨウ スル 1 ステップバイオアッセイマイクロデバイス ノ カイハツ

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Abstract

<p>In this work, an inkjet-printed single-step and homogeneous bioassay microdevice was developed with a poly(dimethylsiloxane) (PDMS) microchannel for easy fabrication and mass production. An inkjet printer can dispense reactive reagents as nanoliter droplets on a PDMS microchannel accurately. Therefore, homogeneous and reproducible dispensing of reagents can be achieved. In a previous report, a complicated fabrication procedure for combining two PDMS microchannel arrays immobilizing two reactive reagents was required. Therefore, in the present report, a new immobilizing method, which involves the patterning of two reactive reagents on two bottom corners of a PDMS microchannel as reagent spots separately based on inkjet printing, was developed. Based on this method, we developed single-step and homogeneous bioassay microdevice that patterns a biotin-sulfonic acid group-introduced graphene oxide conjugate and a fluorescently labeled streptavidin with the same microchannel. For a homogeneous bioassay, the dissolution of immobilized reactive reagents by sample introduction is essential. Therefore, the immobilization matrix, which was mixed with reactive reagents, was investigated. We found that trehalose exhibited favorable characteristics concerning rapid dissolution and uniformity. Then, a single-step detection of biotin was carried out. The detection limit was 0.68 ng mL−1, which was approximately 600-times lower than that of previously reported method. Furthermore, biotin selectivity based on a bioassay microdevice was confirmed. In the future, an immobilization method based on inkjet printing can be applied to various bioassay devices based on the microchannel.</p>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 70 (3), 125-131, 2021-03-05

    The Japan Society for Analytical Chemistry

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