Raman Spectroscopic Assessment of Myocardial Viability in Langendorff-Perfused Ischemic Rat Hearts

• Ikemoto Koki

  Department of Pathology and Cell Regulation, Graduate School of Medical Science, Kyoto Prefectural University of Medicine Department of Cardiovascular Surgery, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

• Hashimoto Kosuke

  Department of Pathology and Cell Regulation, Graduate School of Medical Science, Kyoto Prefectural University of Medicine Present address: Department of Biomedical Chemistry, School of Science and Technology, Kwansei Gakuin University

• Harada Yoshinori

  Department of Pathology and Cell Regulation, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

• Kumamoto Yasuaki

  Department of Pathology and Cell Regulation, Graduate School of Medical Science, Kyoto Prefectural University of Medicine Present address: Department of Applied Physics, Graduate School of Engineering, Osaka University

• Hayakawa Michiyo

  Department of Pathology and Cell Regulation, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

• Mochizuki Kentaro

  Department of Pathology and Cell Regulation, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

• Matsuo Kazuhiko

  Department of Anatomy and Developmental Biology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

• Yashiro Kenta

  Department of Anatomy and Developmental Biology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

• Yaku Hitoshi

  Department of Cardiovascular Surgery, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

• Takamatsu Tetsuro

  Department of Medical Photonics, Kyoto Prefectural University of Medicine

• Tanaka Hideo

  Department of Pathology and Cell Regulation, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

Abstract

Spontaneous Raman spectroscopy, which senses changes in cellular contents of reduced cytochrome c, could be a powerful tool for label-free evaluation of ischemic hearts. However, undetermined is whether it is applicable to evaluation of myocardial viability in ischemic hearts. To address this issue, we investigated sequential changes in Raman spectra of the subepicardial myocardium in the Langendorff-perfused rat heart before and during ligation of the left coronary artery and its subsequent release and re-ligation. Under 532-nm wavelength excitation, the Raman peak intensity of reduced cytochrome c at 747 cm⁻¹ increased quickly after the coronary ligation, and reached a quasi-steady state within 30 min. Subsequent reperfusion of the heart after a short-term (30-min) ligation that simulates reversible conditions resulted in quick recovery of the peak intensity to the baseline. Further re-ligation resulted in resurgence of the peak intensity to nearly the identical value to the first ischemia value. In contrast, reperfusion after prolonged (120-min) ligation that assumes irreversible states resulted in incomplete recovery of the peak intensity, and re-ligation resulted in inadequate resurgence. Electron microscopic observations confirmed the spectral findings. Together, the Raman spectroscopic measurement for cytochrome c could be applicable to evaluation of viability of the ischemic myocardium without labeling.

Journal

• ACTA HISTOCHEMICA ET CYTOCHEMICA

  ACTA HISTOCHEMICA ET CYTOCHEMICA 54 (2), 65-72, 2021-04-28

  JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

Keywords

• Raman spectroscopy
• myocardial viability
• ischemia
• cytochrome c
• mitochondria

Details

• CRID: 1390287838697516032
• NII Article ID: 130008031022
• DOI: 10.1267/ahc.21-00016
• ISSN: 13475800
• ISSN: 00445991
• Web Site: https://www.jstage.jst.go.jp/article/ahc/54/2/54_21-00016/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2022073908
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed